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Next Generation Sequencing Facilitates Quantitative Analysis of CD8+ T cells Transcriptomes from MondoAfl/fl and MondoAfl/flCD4cre Mice

ABSTRACT: RNA-seq of CD8+ T cells from MondoAfl/fl and MondoAfl/flCD4cre Mice

ORGANISM(S): Mus musculus

PROVIDER: GSE276642 | GEO | 2025/05/21

REPOSITORIES: GEO

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Next Generation Sequencing Facilitates Quantitative Analysis of CD8+ T cells Transcriptomes from MondoAfl/fl and MondoAfl/flCD4cre Mice

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of CD8+ T cells Transcriptomes from MondoAfl/fl and MondoAfl/flCD4cre Mice

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Next Generation Sequencing Facilitates Quantitative Analysis of Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl Incisor Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived incisor transcriptome profiling (RNA-seq) between Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl mice to identify the potential target of Runx2. Methods: Incisor mRNA profiles from one week after induction of one-month-old Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl mice were generated by deep sequencing, in triplicate, using Illumina NextSeq500. Results: Using an optimized data analysis workflow, we mapped about 68 million sequence reads per sample to the mouse genome ( mm10) and identified 80,214 transcripts in th incisors of Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl mice with Partek E/M workflow. Five hundred and eleven differentially regulated genes were identified (＞2-fold, p＜0.05), of which 299 were upregulated and 212 were downregulated.

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Next Generation Sequencing Facilitates Quantitative Analysis of Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl Mouse Molar Transcriptomes

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