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Comparison of Different Approaches to Single Cell RNA Sequencing of Cancer Associated Fibroblasts

ABSTRACT: Cancer associated fibroblasts (CAFs) represent a major stromal component of PDAC with both tumor-promoting and restraining properties. Studies have demonstrated functional heterogeneity among different subpopulations of CAFs, highlighting the need to identify specific subpopulations when targeting CAFs. We hereby used two types of processing method, whole tumor or negatively-isolated CAFs, to determine how best to uncover CAF heterogeneity with single-cell RNA sequencing (scRNA-seq).

ORGANISM(S): Mus musculus

PROVIDER: GSE276754 | GEO | 2025/09/14

REPOSITORIES: GEO

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