Dataset Information

Intermittent hypoxia: changing energy metabolism patterns of golden pompano is beneficial for coping with hypoxia

ABSTRACT: It is currently believed that one of the most severe abiotic stresses in the aquatic environment is hypoxia. However, dissolved oxygen in water is not always at the low concentrations that cause hypoxia, but it is possible to exhibit a state of intermittent fluctuation. The study used a low oxygen concentration (1.8 mg/L) to treat golden pompano (Trachinotus blochii) for 28 days (intermittent hypoxia, 2 hours per day) and systematically evaluated its metabolism indexes. RNA-seq was used to construct a regulatory network to further elucidate the transcriptional regulation of the golden pompano metabolism under intermittent hypoxia. We found that the liver lactic acid content, as well as pyruvate kinase, hexokinase, and phosphofructokinase activities were elevated during the first 7 days, suggesting that anaerobic glycolysis was enhanced during the preceding period. In addition, the triglyceride, lipoprotein lipase, and carnitine palmitoyltransferase-1 levels in the liver were elevated after 14 days, suggesting that lipid utilisation was activated after 14 days. At the same time, intermittent hypoxia increases the activity of aspartate aminotransferase and alanine aminotransferase in the liver, decreases total plasma protein and amino acid levels, and enhances the metabolism of protein and amino acids. Decreased levels of oxidative stress and LOEcrit (the O2 tension for loss of equilibrium) in the golden pompano after 28 days of intermittent hypoxia. Transcriptome analysis showed that the fatty acid metabolism, PPAR signalling pathway, fatty acid degradation, D-amino acid metabolism, and cholesterol metabolism pathway were activated. These results suggest that intermittent hypoxia improves the metabolic pattern of golden pompano, enhances its ability to tolerate hypoxia, and promotes its adaptation to hypoxia environment.

ORGANISM(S): Trachinotus blochii

PROVIDER: GSE277111 | GEO | 2026/09/10

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Hypoxia has profound and diverse effects on aerobic organisms, disrupting oxidative phosphorylation and activating several protective pathways. Predictions have been made that exposure to mild intermittent hypoxia may be protective against more severe exposure and may extend lifespan. Here we report the lifespan effects of chronic, mild, intermittent hypoxia and short-term survival in acute severe hypoxia in four clones of Daphnia magna originating from either permanent or intermittent habitats. We test the hypothesis that acclimation to chronic mild intermittent hypoxia can extend lifespan through activation of antioxidant and stress-tolerance pathways and increase survival in acute severe hypoxia through activation of oxygen transport and storage proteins and adjustment to carbohydrate metabolism. Unexpectedly, we show that chronic hypoxia extended the lifespan in the two clones originating from intermittent habitats but had the opposite effect in the two clones from permanent habitats, which also showed lower tolerance to acute hypoxia. Exposure to chronic hypoxia did not protect against acute hypoxia; to the contrary, Daphnia from the chronic hypoxia treatment had lower acute hypoxia tolerance than normoxic controls. Few transcripts changed their abundance in response to the chronic hypoxia treatment in any of the clones. After 12 hours of acute hypoxia treatment, the transcriptional response was more pronounced, with numerous protein-coding genes with functionality in oxygen transport, mitochondrial and respiratory metabolism, and gluconeogenesis, showing up-regulation. While clones from intermittent habitats showed somewhat stronger differential expression in response to acute hypoxia than those from permanent habitats, contrary to predictions, there were no significant hypoxia-by-habitat of origin or chronic-by-acute treatment interactions. GO enrichment analysis revealed a possible hypoxia tolerance role by accelerating the molting cycle and regulating neuron survival through up-regulation of cuticular proteins and neurotrophins, respectively.

Dataset Information

Intermittent hypoxia: changing energy metabolism patterns of golden pompano is beneficial for coping with hypoxia

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets