Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile in Shh-expressing cells of hair follicles in mouse during CIA

ABSTRACT: Chemotherapy-induced alopecia (CIA) is a major unsolved adverse effect in clinical oncology. In previous studies, we proved the Sonic hedgehog(Shh) signaling pathway is a major target of cyclophosphamide (CYP). However, how CYP suppresses Shh gene remains unknown. In this study, we isolated Shh-expressing cells in hair follicles of Shh-GFP+/- mice treated with CYP and tested the effect of CYP on these cells. The result showed that Shh-expressing cells decreased significantly post-CYP treatment. RNA sequencing data showed that compared with the control group, 536 and 2,439 genes expressed differentially at 12hrs and 24hrs respectively post-CYP treatment. KEGG enrichment analysis revealed the p53 and the apoptosis signaling pathway were significantly enriched at 12hrs and 24hrs after CYP injection. The immunofluorescence staining of active Caspase 3 confirmed the apoptosis of Shh-expressing cells at 12 hours post-CYP treatment. GSEA analysis, ChIP assay, and AG-490 rescue assay revealed the IFN/JAK/STAT1 signaling pathway participated in regulating Shh during CIA. The results of this study demonstrated the decrease of Shh during CIA not only because of the loss of Shh-expressing cells but also because of the down expression of Shh in Shh-expressing cells. Moreover, this study also proved that apoptosis is the main reason for the loss of Shh-expressing cells, while the IFN/JAK/STAT1 signaling pathway may play an important role in the downregulation of Shh.

ORGANISM(S): Mus musculus

PROVIDER: GSE277257 | GEO | 2026/03/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	GSE277257_Shh_GFP_CYP_gene_name_counts.txt.gz	Txt

Items per page:

1 - 2 of 2

Similar Datasets

Profiles of cytokines in heart after β-adrenergic stimulation in different time point

Project description:To identify the dynamic changes of cytokines in the heart upon β-adrenergic stimulation (isoproterenol, 5 mg/kg body weight) at 3hrs, 6hrs, 12hrs, 24hrs and 3days using mouse cytokine antibody arrays

2015-09-09 | GSE72799 | GEO

CD24 suppresses malignant phenotype by downregulation of SHH transcription through STAT1 inhibition in breast cancer cells [HCC1937]

Project description:We describe a relationship between CD24 and the Hedgehog (Hh) ligand Sonic Hedgehog (SHH), and reveal a role for this relationship in the induction of a malignant phenotype in breast cancer. Anchorage-dependent proliferation, anchorage-independent proliferation, invasiveness, and tumorigenicity in breast cancer cells (BCCs) transfected with siRNA and plasmid targeting Hh signaling, CD24, and STAT1 were investigated. CD24 siRNA-transfected BCCs demonstrated higher expression of SHH and GLI1, increased anchorage-independent proliferation, enhanced invasiveness and superior tumorigenicity compared with control. Conversely, CD24 forced-expressing BCCs possessed decreased SHH and GLI1 expression, anchorage-independent proliferation, and invasiveness. Suppression of SHH decreased invasiveness through inhibition of matrix metalloproteinase (MMP)-2 expression, GLI1 expression, anchorage-independent proliferation, tumorigenicity, and tumor volume in vivo in CD24 siRNA-transfected BCCs. DNA microarray analysis identified STAT1 as a connection between CD24 and SHH. CD24 siRNA-transfected BCCs with concurrent STAT1 inhibition exhibited decreased SHH expression, invasiveness, anchorage-independent proliferation, tumorigenicity, and tumor volume in vivo. Consistently, STAT1 over-expression induced elevated SHH expression, invasiveness, and anchorage-independent proliferation in BCCs. These results suggest that CD24 suppresses development of a malignant phenotype by down-regulating SHH transcription through STAT1 inhibition.

2015-11-30 | GSE73960 | GEO

CD24 suppresses malignant phenotype by downregulation of SHH transcription through STAT1 inhibition in breast cancer cells [MCF-7]

2015-11-30 | GSE73959 | GEO

Gene expression profile in mouse back skin after cyclophosphamide treatment

Project description:Chemotherapy-induced hair loss (alopecia; CIA) is a highly distressing adverse effect that many cancer patients endure. Yet the molecular pathobiology of CIA remains largely unknown. We analysis the changes of mRNA profiles at different time points after cyclophosphamide treatment using Next-generation Sequencing in a mouse model of CIA. Our result revealed that Shh signaling is an evolutionarily conserved key target in CIA pathobiology.

2018-08-29 | GSE119118 | GEO

Global gene expression analysis of human glioma (Hs 683) cell lines exposed to candoxin

Project description:Total RNA was isolated from candoxin-treated (n = 3 flasks of each time points - 12hrs, 24hrs and 48hrs) and untreated control (n = 3) cells using RNeasy® mini kit.The preparation and processing of labeled, fragmented cRNA for oligonucleotide mcroarray hybridization has been performed according to the manufacturer’s protocols described in the technical manual (Affymetrix, Santa Clara, CA). Keywords: time-course

2005-12-01 | GSE1682 | GEO

Transcription differences of Micrococcus luteus at late-exponential and stationary phase

2012-09-24 | GSE38040 | GEO

Transcription differences of Micrococcus luteus at late-exponential and stationary phase

Project description:Investigation of transcriptomic changes in M.luteus at 12hrs and 24hrs. Differences in fatty acid profiles of M. luteus at exponential and stationary phase is attributed to transcriptional changes of branched amino acid biosynthesis and degradation genes. This study is described by Pereira, J.H., E.B. Goh, J.D. Keasling, H.R. Beller and P.A. Adams in Crystal structure of FabH and factors affecting the distribution of branched fatty acids in Micrococcus luteus, which has been submitted to Acta Crystallographica Section D A 6 microarray study using total RNA recovered from six separate control cultures of Micrococcus luteus NCTC2665 strain with 3 harvested after 12hrs of growth and the other 3 after 24hrs of growth. Each chip measures the expression level of 2,374 ORF based on the draft genome sequence of Micrococcus luteus with ten 60-mer probe pairs (PM/MM) per gene, with 3-fold technical redundancy.

2012-09-24 | E-GEOD-38040 | biostudies-arrayexpress

Dynamic Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery Requires Iron-Sulfur Cluster Incorporation of CIAO3

Project description:Cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) pathway delivers Fe-S clusters to nuclear and cytosolic Fe-S proteins involved in essential cellular functions. This delivery process is regulated by availability of iron and oxygen, but it remains unclear how CIA components orchestrate the cluster transfer under varying cellular environments. Here, we investigated the organization of CIA machinery under various conditions. We developed a targeted proteomics assay monitoring known CIA factors. Using this assay, we were able to detect NUBP1, CIAO3 and CIA substrates in immunoprecipitates of NUBP2, a component of the CIA scaffold complex. We also revealed that NUBP2 transiently associates with the CIA targeting complex (MMS19, CIAO1, CIAO2B), indicating the possible existence of CIA metabolons. We observed stronger interactions between CIAO3 and the CIA scaffold complex upon iron supplementation or low oxygen tension, while iron chelation and reactive oxygen species weaken CIAO3 interactions with CIA components. We further demonstrated that CIAO3 mutant with defective Fe-S cluster binding failed to integrate into the metabolons. However, these mutants unexpectedly exhibit stronger association with CIA substrates regardless of their reduced association with the CIA targeting complex, implicating that CIAO3 and CIA substrates may present in complexes independent of the CIA targeting complex. Together, our data suggested that CIA components potentially form metabolons whose assembly are regulated by environmental cues and require Fe-S cluster incorporation in CIAO3. These findings provided additional evidence that the CIA pathway adapts to changes in cellular environment through complex reorganization.

2021-11-16 | MSV000088394 | MassIVE

circRNA-based biomarker candidates for acute cypermethrin and chlorpyrisfos toxication in the brain of Zebrafish (Danio rerio)

Project description:Cypermethrin (CYP) and chlorpyrifos (CPF) are pesticides which are frequently used in agricultural areas around the world. These chemicals have been shown to cause serious toxicological damage in the brain of fish, which is non-target organisms. However, circRNAs associated with acute brain toxicity caused by cypermethrin and chlorpyrifos have not been studied yet. In this study, circRNAs were identified and characterized using RNA-seq in Zebrafish brains exposed to acute cypermethrin and chlorpyrifos toxicity. A total of 10375 circRNAs were detected. It was determined that 6 circRNAs were up-regulated, 10 circRNAs were down-regulated in CYP brain samples compared to controls . In addition, it was found that 57 circRNAs are up-regulated and 3 circRNAs down-regulated in CPF brain samples compared to controls. Moreover, 62 circRNAs were down-regulated in the CYP samples, when CYP and CPF samples were compared. However, up-regulated circRNA could not be detected. It was revealed that the detected circRNAs specifically regulated the MAPK signaling pathway, endocytosis mechanism, apoptosis and p53 signaling pathway. This study, which was conducted for the first time in terms of the subject of the study, could bring a different perspective especially to pesticide toxicity studies.

2023-12-01 | GSE192669 | GEO

Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

Project description:During limb development, fibroblast growth factors (FGFs) govern proximal-distal outgrowth and patterning. FGFs also synchronize developmental patterning between the proximal-distal and anterior-posterior axes by maintaining sonic hedgehog (SHH) expression in cells of the zone of polarizing activity (ZPA) in the distal posterior mesoderm. SHH, in turn, maintains FGFs in the apical ectodermal ridge (AER) which caps the distal tip of the limb bud. Crosstalk between FGF and SHH signaling is critical for patterned limb development, but the mechanisms underlying this feedback loop are not well characterized. Implantation of FGF beads in the proximal posterior limb bud can maintain SHH expression in the former ZPA domain (evident 3hrs after application), while prolonged exposure (24hrs) can induce SHH outside of this domain. Although temporally and spatially disparate, comparative analysis of transcriptome data from these different populations enriched molecules required for FGF-mediated SHH regulation.

2018-12-18 | GSE114661 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data