Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

INHBA activated PI3K/AKT pathway in gastric cancer cell line MKN45

ABSTRACT: The gastric cancer cell' migration ability, invasion ability and the tumor growth could be promoted by INHBA. To investigate the mechanism underlying the regulation of INHBA in promoting gastric cancer progression, high-throughput sequencing was carried in gastric cancer cell line MKN45.The results showed that PI3K/AKT pathway was activated by INHBA. Which indicated that INHBA regulated gastric cancer progression by activating PI3K/AKT pathway.

ORGANISM(S): Homo sapiens

PROVIDER: GSE277561 | GEO | 2026/03/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from human stomach cell lines (MKN45 and MKN45P)

Project description:Analysis to find splicing variants that are differentially expressed in a highly metastatic stomach cancer cell line, MKN45P, versus its parental cell line, MKN45 Comparison between highly metastatic gastric cancer cell line MKN45P and its parental cell line MKN45

2010-06-08 | E-GEOD-21572 | biostudies-arrayexpress

Affymetrix miRNA chip showed the differential miRNA expression in TRAIL‐resistant BGC823 and TRAIL‐sensitive MKN45 cells

Project description:In order to explore the effect of miRNA on TRAIL sensitivity of gastric cancer, we selected TRAIL-sensitive MKN45 and TRAIL-resistant BGC823 gastric cancer cell line. The expression of miRNA in MKN45 and BGC823 was detected by miRNA chip. "3" is the miRNA expression file of BGC823. "4" is the miRNA expression file of MKN45.

2020-05-24 | E-MTAB-9151 | biostudies-arrayexpress

mRNA sequence Analysis of Apocynin treated MKN45

Project description:Noxo1, a component of NADPH oxidase 1 (NOX1) complex, is upregulated in gastric cancer cells in a inflammation-dependent manner, and plays an important role in tumorigenesis (Oncogene, 33: 3820, 2014). To examine the mechanism of NOX1/ROS signaling in tumorigenesis, MKN45 gastric cancer cells were treated with apocynin, an inhibitor for NOX, and their gene expression was examined by RNA sequencing. Based on expression data, Sox2 was shown to be suppressed by apocynin, suggesting a role of Sox2 in a inflammation-associated gastric tumorigenesis.

2019-01-27 | GSE111520 | GEO

Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

Project description:Gene expression profiles of in vitro selected highly metastatic MKN45-GFP sublines. The results were compared with MKN45-GFP control cell line to determine the metastasis associated genes. Four pairs compared experiment. Each pair was used MKN45-GFP cells as correlated control. Determining on the gene expression trends were by various metastatic ability of each subline.

2011-11-10 | E-GEOD-33570 | biostudies-arrayexpress

Circulating long noncoding RNAs INHBA-AS1, MIR4435-2HG, CEBPA-AS1, and AK001058 in plasma serve as diagnostic markers for gastric cancer

Project description:Background: suitable diagnostic markers for cancers are urgently required in clinical practice. Long noncoding RNAs, which have been reported in many cancer types, are a potential new class of biomarkers for tumor diagnosis. Method: LncRNA gene expression profiles were analyzed in two pairs of human gastric cancer and adjacent non-tumor tissues by microarray analysis. Nine gastric cancer-associated lncRNAs were selected and assessed by quantitative real-time polymerase chain reaction in gastric tissues, and 5 of them were further analyzed in gastric cancer patients’plasma. Results: Five lncRNAs, including AK001058, INHBA-AS1, MIR4435-2HG, UCA1 and CEBPA-AS1 were validated to be increased in gastric cancer tissues. Furthermore, we found that plasma level of these five lncRNAs were significantly higher in gastric cancer patients compared with normal controls. By receiver operating characteristic analysis, we found that the combination of plasma lncRNAs with the area under the curve up to 0.921, including AK001058, INHBA-AS1, MIR4435-2HG, and CEBPA-AS1, is a better indicator of gastric cancer than their individual levels or other lncRNA combinations. Simultaneously, we found that the expression levels of a series of MIR4435-2HG fragments are different in gastric cancer plasma samples, but most of them higher than that in healthy control plasma samples. Conclusion: Our results demonstrate that certain lncRNAs, such as AK001058, INHBA-AS1, MIR4435-2HG, and CEBPA-AS1, are enriched in human gastric cancer tissues and significantly elevated in the plasma of patients with gastric cancer. These findings indicate that the combination of these four lncRNAs might be used as diagnostic or prognostic markers for gastric cancer patients.

2017-01-12 | GSE93512 | GEO

Gene expression data of MKN45 xenograft tumors treated with anti-VEGFR2 and anti-VEGF-A antibodies

Project description:The vascular endothelial growth factor (VEGF) /VEGF receptor (VEGFR) axis is an essential regulator of angiogenesis and important therapeutic target in cancer. However, the efficacy of combination treatment with an anti-VEGFR2 antibody and anti-VEGFR-A antibody on tumor progression is not clear. In this study, we examined the anti-tumor effect of the combination treatment using BALB/c-nu/nu mouse xenograft model subcutaneously injected with human gastric cancer MKN45 cells. In this model, we evaluated intra-tumor molecular changes after the combination treatment by cDNA microarray analysis.

2021-07-28 | GSE160613 | GEO

Changes in gene expression in MKN45 cells in response to Helicobacter pylori lipopolysaccharide

Project description:This study set out to identify global changes in gene expression in MKN45 gastric epithelial cells following 8 hours stimulation with 10 μg/ml lipopolysaccharide (LPS) from the gastric pathogen H. pylori. Microarray analysis was used to compare changes in gene expression between cells treated with 10 μg/ml H. pylori LPS and untreated cells at the same time point. Total RNA was harvested from MKN45 cells following treatment with LPS for 8h or untreated cells at the same time-point. 2 independent experiments were carried out. RNA was labeled and hybridized to GeneChips to analyse changes in gene expression.

2011-01-12 | E-GEOD-25147 | biostudies-arrayexpress

Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

2011-11-10 | GSE33570 | GEO

Changes in gene expression in MKN45 cells in response to Helicobacter pylori lipopolysaccharide

2011-01-12 | GSE25147 | GEO

Exploration the role of INHBA in Hu sheep granulosa cells using RNA-Seq

Project description:Activin/inhibin is an important factor for the fecundity of Hu sheep, and it is involved in follicular development in ovaries. Inhibin subunit beta A (INHBA) participates in the synthesis of activin A and inhibin A. Here we investigated the effect and molecular mechanism of INHBA on cell cycle, apoptosis, activin A/inhibin A secretion in Hu sheep granulosa cells (GCs) based on RNA-Sequencing. A total of 1,687 differentially expressed genes (DEGs) were identified (Fold change ≥ 2 and FDR ≤ 0.01), of which 602 genes were upregulated and 1,087 genes were downregulated in the INHBA interference groups compared with the control groups. Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in the regulation of cell cycle, protein serine/threonine kinase activity and actin cytoskeleton reorganization. Moreover, pathway analysis showed that DEGs were significantly enriched in 40 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including P53 pathway, progesterone-mediated oocyte maturation, PI3K-AKT signaling pathway. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) confirmed the differential expression of 7 selected DEGs. In addition, INHBA knockdown promoted GCs to G1 phase and decreased the S phase cell numbers, initiated GC apoptosis through P53 signal pathway, and decreased the concentration of activin A and inhibin A in medium. Above all, our findings may contribute to a better understanding on the law of follicular development and thus improve the reproductive performance of female animals.

2025-11-12 | GSE218452 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data