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Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis by Inducing Tregs Differentiation to Inhibit Inflammatory Response

ABSTRACT: Psoriasis is an immune-mediated chronic inflammatory skin disease, which is clinically characterized by erythema and scales. The pathogenesis is complex, easy to relapse and difficult to cure. Mesenchymal stem cell exosomes (MSCs) are well known for their potent immunomodulatory properties. However, the possible role and underlying mechanisms of adipose mesenchymal stem cell exosomes (ADSC-EVs) in psoriasis have been rarely studied. Here, we investigate whether ADSC-EVs can alleviate psoriasis-related symptoms. In vivo results indicate that subcutaneous injection of ADSC-EVs can effectively improve imiquimod (IMQ)-induced psoriasis in mice. miRNA sequencing analysis aimed to obtain differentially expressed miRNAs in ADSC-EVs and IL17A-EVs, and found that hsa-miR-21-5p, hsa-miR-10a-5p and hsa-miR-126-5p were up-regulated in ADSC-EVs. For further mechanism research, we used exosomes and miRNA to treat T cells respectively to detect changes in the proportion of Treg cells and inflammation-related factors. The results revealed that ADSC-EVs and hsa-miR-21-5p, hsa-miR-10a-5p and hsa-miR-126-5p can induce Treg differentiation, inhibit the secretion of pro-inflammatory factors and promote the secretion of anti-inflammatory factors. The same conclusion was reached by administering ADSC-EVs in IMQ psoriasis mice. These findings indicated that ADSC-EVs alleviated psoriasis inflammation possibly by promoting Treg differentiation through hsa-miR-21-5p, hsa-miR-10a-5p and hsa-miR-126-5p. This study provides new light on the application of ADSC-EVs as a promising therapeutic method for psoriasis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE277657 | GEO | 2026/09/20

REPOSITORIES: GEO

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Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis by Inducing Tregs Differentiation to Inhibit Inflammatory Response

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