Dataset Information

Transcriptional response of Candida albicans to the utilization of aromatic compounds as the sole carbon source

ABSTRACT: Main goal of this analysis was to idnetify metabolic adaptation processes in C. albicans when aromatic compounds, like catechol or hydroquinone are used as the sole carbon source (C-source) and how this is mediated on the transcriptional level. Further, a suspected regulatory activity of the transcription factors Stp2, a central regulator of amino acid metabolism, and Zcf25, a putative regulator of the oxoadipate pathway, on these processes was investigated. Therefore, the SC5314 wild type and single and dual knockout strains of the aforementioned regulators (stp2Δ, zcf25Δ, stp2Δ/zcf25Δ) were incubated in medium containing either glucose (SD), a mixture of proteinogenioc amino acids (SC), catechol (CAT) or hydroquinone (HQN) as the sole C-source and the cellular transcriptome investigated via RNA-Sequencing to define trasncriptional changes in response to the indicated different C-sources.

ORGANISM(S): Candida albicans

PROVIDER: GSE277771 | GEO | 2025/10/07

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Its characteristic rose-like aroma makes phenylethanol a popular ingredient in foods, beverages and cosmetics. Microbial production of phenylethanol currently relies on whole-cell bioconversion of phenylalanine with yeasts that harbor an Ehrlich pathway for phenylalanine catabolism. Complete biosynthesis of phenylethanol from a cheap carbon source such as glucose provides an economically attractive alternative for phenylalanine bioconversion. In this study, a Synthetic Genetic Array screening was applied to identify genes involved in regulation of phenylethanol synthesis in Saccharomyces cerevisiae. The screen focused on transcriptional regulation of ARO10, which encodes the major decarboxylase involved in conversion of phenylpyruvate to phenylethanol. A deletion in ARO8, which encodes an aromatic amino acid transaminase, was found to cause a transcriptional upregulation of ARO10 during growth with ammonium sulfate as the sole nitrogen source. Physiological characterization revealed that the aro8M-oM-^AM-^D mutation led to substantial changes in the absolute and relative intracellular concentrations of amino acids. Moreover, deletion of ARO8 led to de novo production of phenylethanol during growth on a glucose synthetic medium with ammonium as the sole nitrogen source. The aro8 mutation also stimulated phenylethanol production when combined with other, previously documented mutations that deregulate aromatic amino acid biosynthesis in S. cerevisiae. The resulting engineered S. cerevisiae strain produced over 3 mM of phenylethanol from glucose during growth on a simple synthetic medium. The strong impact of a transaminase deletion on intracellular amino acid concentrations opens new possibilities for yeast-based production of amino acid-derived products. The goal of the present study was to identify genes that influence the transcriptional (de)repression of the Ehrlich pathway during growth with ammonium as the nitrogen source. With the aid of Synthetic Genetic Array technology, we constructed a strain collection in which deletions in the non-essential genes in the S. cerevisiae genome were combined with a reporter plasmid comprising the ARO10 promoter fused to a reporter gene (egfp) encoding a fluorescent reporter protein. After screening by flow cytometry, deletion of ARO8 led to a deregulated expression from the ARO10 promoter. The impact of this deletion was further studied by transcriptome and intracellular metabolite analyses. Furthermore, phenylethanol production was measured in strains that combined the aro8 mutation with mutations that were previously shown to deregulate aromatic amino acid biosynthesis.

Dataset Information

Transcriptional response of Candida albicans to the utilization of aromatic compounds as the sole carbon source

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets