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Rhythmic Transcription and Aging: _x000B_Insights from the Drosophila Photoreceptor Transcriptome and Epigenome

ABSTRACT: Rhythmic changes in the transcriptome are widespread during aging in Drosophila photoreceptors. To better understand the epigenetic factors underlying these changes, we profiled RNA PolII occupancy and levels of H3K4me1, H3K4me2, and H3K4me3 using CUT&RUN. To profile changes in chromatin accessibility, we also performed ATAC-seq. To obtain robust rhythmic data across age, we collected both young (D10 post-eclosion) and old (D50 post-eclosion) flies every four hours throughout the day in a 12-hour light:12-hour dark cycle in triplicate.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE278041 | GEO | 2025/12/31

REPOSITORIES: GEO

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