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GSC vs Kc

ABSTRACT: This series compares gene expression between germ line stem cells (GSCs) purified either from bam mutant or Dpp-overexpressing ovaries, with gene expression in Kc cells Keywords: bam mutant, Dpp overexpression

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE2783 | GEO | 2005/06/15

SECONDARY ACCESSION(S): PRJNA92323

REPOSITORIES: GEO

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Transcription profiling of Drosophila melanogaster germ line stem cells from bam mutant or Dpp overexpressing ovaries vs Kc cell controls

Project description:This series compares gene expression between germ line stem cells (GSCs) purified either from bam mutant or Dpp-overexpressing ovaries, with gene expression in Kc cells Experiment Overall Design: 3 replicates of GSCs from bam mutant ovaries Experiment Overall Design: 3 replicates of GSCs from Dpp overexpressing ovaries Experiment Overall Design: 3 replicates of Kc tissue culture cells

2007-10-31 | E-GEOD-2783 | biostudies-arrayexpress

GSC differentiation-reversion test

Project description:gene expression is tested for an association with GSCs by comparing expression levels in ovary tips of c587-GAL4; hs-bam UAS-dpp females at 0 hr, 20 hr and 50 hr after heat shock ; results of two independent experiments are given as expt 1 and expt 2 Keywords: time course, heat shock, bam overexpression

2005-06-15 | GSE2784 | GEO

Transcription profiling of Drosophila melanogaster BAM over expressing germline stem cells vs controls

Project description:Gene expression is tested for an association with GSCs by comparing expression levels in ovary tips of c587-GAL4; hs-bam UAS-dpp females at 0 hr, 20 hr and 50 hr after heat shock ; results of two independent experiments are given as expt 1 and expt 2 Experiment Overall Design: 0 hr ovary tips contain many GSCs Experiment Overall Design: 20 hr hs-bam has induced differentiation of all GSCs Experiment Overall Design: 50 hr Bam has turned over and cystocytes have reverted to become GSCs again Experiment Overall Design: see Kai et al. (2005) Dev. Biol, in press.

2007-11-02 | E-GEOD-2784 | biostudies-arrayexpress

Transcription profiling of Drosophila ovaries from bam mutant and stwl bam double mutant females

Project description:Abstract: During Drosophila oogenesis, germline stem cell (GSC) identity is maintained largely by preventing the expression of factors that promote differentiation. This is accomplished via the activity of several genes acting either in the GSC or its niche. The translational repressors, Nanos and Pumilio, act in GSCs to prevent differentiation, likely by inhibiting translation of early differentiation factors, while niche signals prevent differentiation by silencing transcription of the differentiation factor Bam. We have found that the DNA-associated protein Stonewall (Stwl) is also required for GSC maintenance. stwl is required cell-autonomously; clones of stwl- germ cells were lost by differentiation, and ectopic Stwl caused an expansion of GSCs. stwl mutants acted as Suppressors of Variegation, indicating stwl normally acts in chromatin-dependent gene repression. In contrast to several previously described GSC maintenance factors, Stwl likely functions epigenetically to prevent GSC differentiation. Stwl-dependent transcriptional repression does not target bam, but rather Stwl represses the expression of many genes, including those that may be targeted by Nanos/Pumilio translational inhibition. Experiment Overall Design: Genetic variation comparison between germ cells of Drosophila ovaries from bam mutant and stwl bam double mutant females. Six total samples were analyzed, with each genotype performed in triplicates.

2008-03-13 | E-GEOD-6928 | biostudies-arrayexpress

Molecular profiling of stem cell-like female germ line cells in Drosophila delineates networks important for stemness and differentiation

Project description:We characterize the transcriptome of germline stem cell (GSC)-like cells isolated from bag of marbles (bam) mutant Drosophila ovaries by next generation RNA sequencing (RNA-seq) and compare it to the transcriptome of germline cells isolated from wild type ovaries. We further refine this dataset by utilizing an RNA-immunoprecipitation strategy to identify transcripts bound to the master differentiation factor Bam.

2019-10-31 | GSE138987 | GEO

A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction [hiPSC-EMP-KC-pKC]

Project description:To incorporate Kupffer cells into hiPSC-LOs, we differentiated erythro-myeloid progenitors (EMPs) from hiPSCs. We compares the gene expression profiles of hiPSC-EMPs with cord-blood hematopoietic stem and progenitor cells (CB-HSPCs); and EMP-generated Kupffer cells (EMP-KC) with human primary Kupffer cells.

2024-03-01 | GSE233538 | GEO

Drosophila melanogaster

Project description:Expression data from bam and setdb1 mutant ovaries

| PRJNA312580 | ENA

Identification of Dpp target genes during the early embryo development

Project description:The dorsal patterning in Drosophila is controlled by an extracellular gradient of the morphogens Decapentlaplegic/Screw (Dpp/Scrw), which are members of BMP/TGF-β family. Dpp/Scrw signal is transduced to the nucleus by the transcription factors, Mad/Medea. The transcriptional effectors exert their regulation in a graded-manner eliciting at least three threshold responses: high, intermediate and low. However, the mechanism underlying differential response to Dpp is poorly understood, due in part to the insufficient number of well-studied target genes. Gene expression changes were analysed in ectopic overexpressing Dpp mutant embryos to identify new target genes of Dpp/Mad pathway.

2016-02-24 | GSE78226 | GEO

Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila

Project description:Germline stem cells (GSCs) self-renew and differentiate to sustain a continuous production of gametes. In the female Drosophila germ line, two differentiation factors, bag of marbles (bam) and benign gonial cell neoplasm (bgcn), work in concert in the stem cell daughter to promote the generation of eggs. In GSCs, bam transcription is repressed by signaling from the niche and is activated in stem cell daughters. In contrast, bgcn is transcribed in both the GSCs and stem cell daughters, but little is known about how bgcn is transcriptionally modulated. Here, we find that the conserved protein Nipped_A acts through the Tat interactive protein 60kDa (Tip60) Histone acetyl transferase (HAT) complex in the germ line to promote GSC differentiation. We find that Nipped_A is required for efficient exit from the gap phase 2 (G2) of cell cycle of the GSC daughter and for expression of differentiation factor bgcn. Loss of Nipped_A results in accumulation of GSC daughters. Forced expression of bgcn, in Nipped_A germline-depleted ovaries rescues this differentiation defect. Together, our results indicate that Tip60 complex coordinates cell cycle progression and expression of bgcn to help drive GSC daughters toward a differentiation program.

2018-09-01 | GSE119328 | GEO

Expression data from gonads of Meioc+/+ and Meioc-/-mice

Project description:Meiosis is a specialized division that is specifically engaged in germ cells. This program is finely and dichotomically regulated: germ cells enter meiosis at fetal life in ovaries (13.5 dpc) and only at post-natal life in testes (8 dpp). We used microarrays to determine the gene expression modifications in Meioc mutant gonads displaying early meiotic defects. 14.5 dpc ovaries and 8 dpp testes from Meioc+/+ and Meioc-/- mice (NMRI) were dissected and processed for RNA extraction and hybridization on Affymetrix microarrays. Two pools were analysed for each genotype and each sex. We sought to constitutes pairs (A and B) of wild type and knock-out pools from embryos and pups that were homogenous for developmental stages and from matched littermates. 10 ovaries from 5 embryos composed each pool for females and 4 testes from 4 pups composed each pool for males.

2015-11-19 | E-GEOD-66880 | biostudies-arrayexpress

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