Protect and shield strategy of reactive acyl glucuronides licenses precision-omics investigations into drug-induced liver injury
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE278317 | GEO | 2026/01/31
REPOSITORIES: GEO
Similar Datasets
Project description:Protect and shield strategy of reactive acyl glucuronides licenses precision-omics investigations into drug-induced liver injury
| PRJNA1166320 | ENA
Project description:The aim of this study is to discover genes regulated by miR-204. Differential gene expression in HEK-293 cells transfected with miR-204-mimic compared to HEK-293 cells transfected with control oligo (HEK-293 control) was analyzed using the Agilent Human Whole Genome 4x44K gene expression array (Agilent Technologies, Santa Clara, CA).
2012-03-31 | GSE28400 | GEO
Project description:Interactors of human ZUFSP in HEK-293T cells. Flp-in T-REx 293 cells with stable 3xFLAG-ZUFSP integration were assayed for ZUFSP interactors under three different conditions: i) Cells expressing wild-type ZUFSP, ii) Cells expressing catalytically inactive ZUFSP, iii) Cells expressing wild-type ZUFSP with additional treatment by an unspecific nuclease (Benzonase) during purification.
2018-04-06 | PXD008731 | Pride
Project description:Mitochondrial proteases regulate the dynamic properties of the organelle morphology and ensure functional plasticity at the cellular level. The metalloprotease OMA1 mediates constitutive and stress-inducible processing of its substrates in mitochondria, but the number of functionally characterized substrates remains limited. Using multiproteomic and biochemical approaches, we show that the membrane-anchored inner membrane space (IMS) protein AIFM1 serves as a mitochondrial stress-responsive substrate of OMA1. We define that OMA1 cleaves AIFM1 in the IMS under stress conditions, which is a kinetically slower reaction than that of the conventional substrate, the dynamin-like GTPase OPA1. Membrane dislocation of cleaved AIFM1 in mitochondria reduces its binding to subunits of the oxidative phosphorylation machinery. This leads to a decrease in the respiratory activity and ultimately impairs cell growth. Mechanistically, we show that AIFM1 broadly safeguards the mitochondrial proteome at steady state by participating in the protein import, in particular respiratory complex I subunits, via the Tim23 complex. These results reveal an unrecognized role for OMA1 in integrating mitochondrial stress sensing and cellular energetics by altering the topology of AIFM1.The internal identifier was: m4m1hgAENCThe repository contains AIFM1 wildtype and KO TREx cells (samples 1-8), which were not used in the associated publication. The file names contain pp for +/+ and mm for -/-.File names 09-12 (file names: R98ENLYFG) correspond to TCS. The file names 13-16 (M1_R98del) correspond to TCS/TEV.Raw fileSample Name01_AIFM1_KO_pp_fyxK.rawFlp-In-T REx-293-mock#102_AIFM1_KO_pp_wxP9.rawFlp-In-T REx-293-mock#203_AIFM1_KO_pp_Djpr.rawFlp-In-T REx-293-mock#304_AIFM1_KO_pp_txOk.rawFlp-In-T REx-293-mock#405_AIFM1_KO_mm_moaT.rawFlp-In-T REx-293 AIFM1 KO-mock#106_AIFM1_KO_mm_W5lK.rawFlp-In-T REx-293 AIFM1 KO-mock#207_AIFM1_KO_mm_XIc0.rawFlp-In-T REx-293 AIFM1 KO-mock#308_AIFM1_KO_mm_pdxt.rawFlp-In-T REx-293 AIFM1 KO-mock#409_AIFM1_C_MycAIFM1_R98ENLYFG_I2x8.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS#110_AIFM1_C_MycAIFM1_R98ENLYFG_Hzqq.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS#211_AIFM1_C_MycAIFM1_R98ENLYFG_4P4M.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS#312_AIFM1_C_MycAIFM1_R98ENLYFG_MktX.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS#413_AIFM1_C_MycAIFM1_M1_R98del_7NiF.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS/TEV#114_AIFM1_C_MycAIFM1_M1_R98del_yf68.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS/TEV#215_AIFM1_C_MycAIFM1_M1_R98del_hmKM.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS/TEV#316_AIFM1_C_MycAIFM1_M1_R98del_qXgO.rawFlp-In-T REx-293 AIFM1 KO-AIFM1TCS/TEV#4
2026-01-31 | PXD063602 | Pride
Project description:Human embryonic kidney (HEK) cells 293 are analyzed by two state-of-the-art MS instruments (Thermo Orbitrap Fusion and Q Exactive HF) in quadruplicate for precision evaluation.
2018-05-08 | PXD009720 |
Project description:The aim of this study is to discover genes regulated by miR-204. Differential gene expression in HEK-293 cells transfected with miR-204-mimic compared to HEK-293 cells transfected with control oligo (HEK-293 control) was analyzed using the Agilent Human Whole Genome 4x44K gene expression array (Agilent Technologies, Santa Clara, CA). HEK-293 cells were transfected with either miR-204 or a control, and gene expression was analyzed using the Agilent Human Whole Genome 4x44K array. A dye-swap was performed.
2012-03-30 | E-GEOD-28400 | biostudies-arrayexpress
Project description:Transcriptional profiling of 1μg/mL dox and 5μM 1NMPP1 treatedT-REx 293 cells expressing either WT or I642G IRE1α vs control.
2014-12-22 | GSE17152 | GEO
Project description:We used high-throughput sequencing to investigate the genome-wide transcriptional response in human cells to treatment with Borrelia burgdorferi. We chose a time point of 72 h as ticks feed on their host for several days and at the same time the early response in Lyme disease is expected to occur at this time period at a cellular level. We found that the two cell models studied (HUVEC and HEK-293 cells) had significantly different responses. More significantly differentially expressed genes (69 in total) were found in HUVEC cells than in HEK-293 cells (8 in total). Functional analysis indicated induction of the immune response in HUVEC and suggest changes in the extracellular matrix in HEK-293.
2024-09-30 | GSE194294 | GEO
Project description:Human embryonal kidney cells (HEK-293) are the most common host cells used for transient recombinant adeno-associated virus (rAAV) production in pharmaceutical industry. To better cover the expected gene therapy product demands in the future, different traditional strategies such as cell line sub-cloning and/or addition of chemical substances to the fermentation media have been used to maximize titers and improve product quality. A more effective and advanced approach to boost yield can be envisaged by characterizing the transcriptome of different HEK-293 cell line pedigrees with distinct rAAV productivity patterns to subsequently identify potential gene targets for cell engineering. In this work, the mRNA expression profile of three HEK-293 cell lines, resulting in various yields during a fermentation batch process for rAAV production, was investigated to gain basic insight into cell variability and eventually to identify genes that correlate with productivity. Mock runs using only transfection reagents were performed in parallel as a control. We found significant differences in gene regulatory behaviors between the three cell lines at different growth and production stages. The evaluation of these transcriptomics profiles combined with collected in-process control parameters and titers shed some light on potential cell engineering targets to maximize transient production of rAAV in HEK-293 cells Comparison of three HEK-293 suspension cell lines transcriptomics during an AAV production process
2024-05-13 | GSE226054 | GEO
Project description:Flp-In T-REx-293 cells transfected with non-targeting control siRNA or UPF1-specific siRNA as indicated and used for total RNA-seq.
2022-04-07 | GSE176197 | GEO