Transcriptomics

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Single cell analysis of an engineered organoid based model of pancreatic cancer identifies hypoxia as a contributing factor in the determination of transcriptional subtypes


ABSTRACT: Pancreatic ductal adenocarcinoma is a high-mortality cancer with an aggressive, treatment resistant phenotype and a complex tumour microenvironment featuring significant hypoxia. We leveraged a unique 3D in vitro platform (TRACER) to perform single-cell transcriptome analysis of organoids cultured in a spatially-defined microenvironmental gradient to explore the effect of oxygen and other microenvironmental gradients on organoid heterogeneity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE278477 | GEO | 2025/07/09

REPOSITORIES: GEO

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