Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Transcriptional and Cellular Exploration of Obesity and Type-II Diabetes in Human Adipose Tissues [scRNA-seq]

ABSTRACT: Obesity is tightly linked to metabolic disorders such as type-II diabetes (T2D), though a sub-population of individuals with obesity do not present with such morbidities. The biological factors conferring T2D vulnerability remain incompletely understood. We recruited 58 individuals with obesity with or without a clinical diagnosis of T2D (Ob+T2D and Ob). We profiled paired samples of omental and subcutaneous adipose by scRNA-seq (n=10), flow cytometry under basal and stimulated conditions (n=42), and histology (n=17). Flow cytometry and chemokine quantification were also performed in paired whole blood. We identified depot-specific reprogramming of the omental non-haematopoietic compartment in Ob+T2D, centred on mesothelial, endothelial, innate T cells, and macrophages. Mesothelial and IGFBP2+ cells exhibited a fibrotic, EMT-like phenotype, while omental endothelial cells exhibited a lipid-overload, inflammatory, antigen presentation profile in T2D. Omental and subcutaneous adipose innate T cells exhibited an inflammatory profile akin to that identified in mice to temper macrophage inflammation during obesity. These findings reveal new insights into the pathophysiology of T2D in adipose tissue and identify compensatory networks within the tissues that could be exploited by targeted therapeutics.

ORGANISM(S): Homo sapiens

PROVIDER: GSE278526 | GEO | 2026/06/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression differences in omental mesothelial cells from lean and obese human donors

Project description:Visceral adipose tissue inflammation correlates with metabolic dysfunction in obesity. However, critical cell types that trigger and contribute to inflammation in this depot are not completely understood. Mesothelial cells line the omental fat depot and are thought to contribute to several intraperitoneal inflammatory conditions. Whether adipose-associated mesothelial cell inflammation is a feature of pathologic obesity is unclear. This study was performed to delineate a contribution of omental adipose mesothelial cells to visceral adipose tissue dysfunction in pathologic obesity. We performed a cross-sectional case-control study from two separate cohorts of patients undergoing abdominal surgery with varying degrees of obesity and metabolic disease. Whole-genome expression profiling was conducted on mesothelial cells isolated from lean and obese surgical patients to determine differences in relevant pathways related to obesity and metabolic disease.

2019-03-08 | GSE128021 | GEO

miRNA profiling by array of human paired adipose tissue (subcutaneous and omentum) before and after gastric-bypass and weight loss

Project description:Objective: Adipose tissue plays a key role in obesity related metabolic dysfunction. MicroRNA (miRNA) are gene regulatory molecules involved in inter-cellular and inter-organ communication. We hypothesised that miRNA levels in adipose tissue would change after gastric bypass surgery and that this would provide insights into their role in obesity-induced metabolic dysregulation. Methods: miRNA-profiling (Affymetrix_Gene-Chip_miRNA2.0_Arrays) of omental and subcutaneous adipose (n=15 females) before, and after, gastric bypass surgery. Results: One omental, and thirteen subcutaneous adipose miRNAs were significantly, differentially expressed after gastric bypass, including down-regulation of miR-223-3p and its antisense relative, miR-223-5p, in both adipose tissues. mRNA levels of miR-223-3p targets NLRP3 and GLUT4 were increased and decreased respectively following gastric bypass in both adipose tissues. Significantly more NLRP3 protein was observed in omental adipose after gastric bypass (P=0.02). Significant hypomethlyation of NLRP3 and hypermethylation of miR-223 was observed in both adipose tissues after gastric bypass. In subcutaneous adipose significant correlations were observed between both miR-223-3p and miR-223-5p and glucose, and between NLRP3 mRNA and protein levels and blood lipids. Conclusions: This is the first report detailing genome-wide miRNA-profiling of omental adipose before and after gastric bypass, and further highlights a link between miR-223-3p and the NLRP3 inflammasome in obesity.

2019-12-16 | E-MTAB-8594 | biostudies-arrayexpress

FKBP5 expression in human adipose tissue increases following dexamethasone exposure and is associated with insulin resistance.

Project description:Glucocorticoid excess is linked to central obesity, adipose tissue insulin resistance and type 2 diabetes mellitus. The aim of our study was to investigate the effects of dexamethasone on gene expression in human subcutaneous and omental adipose tissue, in order to identify potential novel mechanisms and biomarkers for glucocorticoid-induced insulin resistance in adipose tissue. Dexamethasone changed the expression of 527 genes in both subcutaneous and omental adipose tissue. FKBP5 and CNR1 were the most responsive genes in both depots (~7-fold increase). Dexamethasone increased FKBP5 gene and protein expression in a dose-dependent manner in both depots, but FKBP5 protein levels were 10-fold higher in omental than subcutaneous adipose tissue. FKBP5 gene expression in subcutaneous adipose tissue was positively correlated with serum insulin, HOMA-IR and subcutaneous adipocyte diameter, while fold change in gene expression by dexamethasone was negatively correlated with clinical markers of insulin resistance, i.e. HbA1c, BMI, HOMA-IR and serum insulin. Only one gene, SERTM1, clearly differed in response to dexamethasone between the two depots. Dexamethasone at high concentrations, influences gene expression in both subcutaneous and omental adipose tissue in a similar pattern and promotes gene expression of FKBP5, a gene that may be implicated in glucocorticoid-induced insulin resistance. Paired human subcutaneous (sc) and omental (om) adipose tissue samples obtained from 4 non-diabetic adipose tissue donors (4 M; BMI: 20.8-27.5 Kg/m2) were incubated without (Ctr) or with dexamethasone (Dex, 3 M-NM-<M) for 24 h.

2014-08-18 | E-GEOD-42902 | biostudies-arrayexpress

Gene expression analysis of subcutaneous and omental adipose tissue from obese, insulin-resistant women

Project description:The association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance. Subcutaneous and omental adipose tissue and serum were obtained from 29 obese nondiabetic women, 13 of whom were hyperinsulinemic. Histology, and lipid and gene profiling were performed. In omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in GM3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, PEMT, decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum. Total RNA was isolated and up to 400 ng of total RNA per sample was labelled and hybridized to Illumina HumanHT-12_V4 expression BeadChip platform. Paired subcutaneous and omental samples from 6 women were analysed.

2015-12-07 | E-GEOD-73108 | biostudies-arrayexpress

FKBP5 expression in human adipose tissue increases following dexamethasone exposure and is associated with insulin resistance

2014-08-18 | GSE42902 | GEO

Human Illumina 850K EPIC methylation arrays of blood, subcutaneous and omental visceral adipose tissue from two-step surgical approach

Project description:Genome wide DNA methylation in blood, subcutaneous and omental visceral adipose tissue from two-step surgical approach (N=9) was analysed in patients with severe obesity using Illumina 850K EPIC technology before and after metabolic surgery (Leipzig Obesity BioBank (LOBB) cohort). Additionally, a validation blood cohort of patients with obesity undergoing metabolic surgery was analyzed for results validation.

2024-03-31 | E-MTAB-13564 | biostudies-arrayexpress

Gene expression analysis of subcutaneous and omental adipose tissue from obese, insulin-resistant women

2015-12-07 | GSE73108 | GEO

Expression data from human omental and subcutaneous adipose tissue taken from volunteers undergoing bariatric surgery

Project description:This SuperSeries is composed of the following subset Series: GSE29409: Subcutaneous and omental white adipose tissue biopsies analysed from five obese patients GSE29410: Subcutaneous and omental white adipose tissue biopsies analysed from three obese patients Refer to individual Series

2012-01-01 | E-GEOD-29411 | biostudies-arrayexpress

Methylation of different human adipose tissues

Project description:Characterization of human epiploic adipose tissue (AT), compared to other AT: omental, mesenteric and subcutaneous. AT was surgically removed, collected and methylome as well as proteome and transcriptome was analyzed. For 12 donors, DNA methylation was analyzed for all 4 aforementioned AT types, while 2 donors contributed one and 3 types, resp. Donors showed obesity, but were either insulin sensitive or insulin resistant.

2021-11-01 | E-MTAB-10999 | biostudies-arrayexpress

Expression data from human adipose tissue

Project description:Obesity is a risk factor for numerous metabolic disorders; however, not all obese individuals are prone to insulin resistance. The central aim of this study was to identify molecular pathways directly related to insulin resistance independent of BMI in obesity. We sought to determine the gene expression signature of adipose tissue in a body mass index (BMI)-matched obese cohort of patients that are either insulin sensitive or insulin resistant. We determined the global gene expression signatures of omental and subcutaneous adipose tissue samples obtained from insulin-sensitive obese and insulin-resistant obese patients undergoing gastric bypass surgery.

2009-04-24 | E-GEOD-15773 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data