Transcriptomics

Dataset Information

17

MiRNA profiling of Adenosine treated Endothelial Progenitor Cells (EPCs)


ABSTRACT: Studies of miRNA profiling in early and late endothelial progenitor cells treated or not by cardioprotective nucleoside adenosine. Overall design: Early outgrowth endothelial progenitor cells were obtained by adhesion of peripheral blood mononuclear cells of healthy volunteers. Late endothelial progenitor cells were obtained by purification of CD34+ peripheral blood cells and were cultured and amplified in endothelial-specific medium containing growth factors. Both cell types were treated by adenosine (10micromol/L) for 6 hours. Total RNA was extracted using mirVana Kit and quantified by Nanodrop. RNA was labeled and hybridized using Agilent miRNA Complete Labeling and Hyb Kit. 3 to 4 arrays per sample were hybridized and scanned with the Genepix 4000B Scanner (Molecular Devices). Six independent experiments were performed.

INSTRUMENT(S): Agilent-021827 Human miRNA Microarray (V3) (Probe Name version)

ORGANISM(S): Homo sapiens  

SUBMITTER: Yvan Devaux   

PROVIDER: GSE27874 | GEO | 2011-03-15

SECONDARY ACCESSION(S): PRJNA138007

REPOSITORIES: GEO

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