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The cellular response to the deprivation of L-phenylalanine from the medium in the cultivation of A375, a melanoma cell line.

ABSTRACT: To optimize the efficacy of boron neutron capture therapy (BNCT), we investigated the optimal conditions for maximizing the uptake of boronophenylalanine (BPA), an analog of L-phenylalanine (L-Phe), in tumor cells. In the cultivation of tumor cell lines, some cells showed an amplified accumulation of BPA under 24 hours of L-Phe deprivation, which resulted in an enhancement of BNCT efficiency. In contrast, A375 demonstrated unaltered responsiveness in the uptake of BPA under L-Phe restriction. Accordingly, we conducted a comprehensive analysis of the cellular response of A375 cells under L-Phe restriction by RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE279290 | GEO | 2025/12/31

REPOSITORIES: GEO

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The cellular response to the deprivation of L-phenylalanine from the medium in the cultivation of HaCaT, a keratynocyte cell line.

Project description:To optimize the efficacy of boron neutron capture therapy (BNCT), we investigated the optimal conditions for maximizing the uptake of boronophenylalanine (BPA), an analog of L-phenylalanine (L-Phe), in tumor cells. In the cultivation of tumor cell lines, some cells showed an amplified accumulation of BPA under 24 hours of L-Phe deprivation, which resulted in an enhancement of BNCT efficiency. In contrast, HaCaT, a keratynocyte cell line, demonstrated unaltered responsiveness in the uptake of BPA under L-Phe restriction. Accordingly, we conducted a comprehensive analysis of the cellular response of HaCaT cells under L-Phe restriction by RNA-seq.

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Proteomic characterization of SAS-derived extracellular vesicles following treatment with BPA and doses of neutron irradiation

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Transcriptome Profiling of Black Poplar under Boron Toxicity Revealed Genetic Regulation of Boron Uptake, Transport and Detoxification

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