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Identification of differentially regulated genes upon shRNA-mediated knock-down of HLX in the URE leukemia cell line

ABSTRACT: To study the role of Hlx in hematopoietic differentiation and tumorigenesis, URE cells were infected with short-hairpin-containing pSIH1-H1-copGFP lentiviral vector (System Biosciences, Mountain View, CA) containing either nucleotide sequences targeting luciferase (shControl) or HLX (shHLX). After 24hrs incubation in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene, cells were cultured in fresh medium for several days. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared.

ORGANISM(S): Mus musculus

PROVIDER: GSE27939 | GEO | 2012/09/14

SECONDARY ACCESSION(S): PRJNA141721

REPOSITORIES: GEO

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Identification of differentially regulated genes upon shRNA-mediated knock-down of HLX in the URE leukemia cell line

Project description:To study the role of Hlx in hematopoietic differentiation and tumorigenesis, URE cells were infected with short-hairpin-containing pSIH1-H1-copGFP lentiviral vector (System Biosciences, Mountain View, CA) containing either nucleotide sequences targeting luciferase (shControl) or HLX (shHLX). After 24hrs incubation in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene, cells were cultured in fresh medium for several days. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared. URE cells were infected with short-hairpin-containing pSIH1-H1-copGFP lentiviral vector (System Biosciences, Mountain View, CA) containing either nucleotide sequences targeting luciferase (shControl) or HLX (shHLX). After 24hrs incubation in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene, cells were cultured in fresh medium for several days. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared. Three replicates of each, shControl and shHLX-transduced cells were used. The goal was to study the role of Hlx in hematopoietic differentiation and tumorigenesis.

2012-09-14 | E-GEOD-27939 | biostudies-arrayexpress

Identification of differentially regulated genes upon overexpression of HLX in wild-type hematopoietic stem cells

Project description:The goal was to study the role of Hlx in hematopoiesis. Sorted Lin-Kit+Sca-1+ cells from wild-type FVB/nJ bone marrow were infected with control (pCAD-IRES-GFP) or Hlx lentivirus (pCAD-IRES-GFP-Hlx) and cultured for 2 days in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared. Three replicates of each, control vector transduced and HLX-transduced cells were used.

2012-09-14 | E-GEOD-27938 | biostudies-arrayexpress

Genes upregulated by HLX

Project description:HLX was found as a VEGF-A induced gene in HUVEC (B.Schweighofer, submitted). In order to detect genes regulated by HLX HUVEC were infected by recombinant adenovirus expressing HLX for 4, 8, 16 and 32h. RNA was isolated and subjected to microarray analysis using Affymetrix microarray. HUVEC were infected with a MOI of 40 with either HLX expressing or empty adenovirus for 4, 8, 16 or 32 hours. HUVEC without an adenovirus infection were used as control.Total RNA was isolated with Trizol according to the instructions of the manufacturer. Gene expression was analysed with Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays according to the Affymetrix protocol.

2011-02-15 | E-GEOD-13054 | biostudies-arrayexpress

Genes upregulated by HLX

2011-02-15 | GSE13054 | GEO

Gene expression analysis of leukemia-initiating cells of compound URE-/+::Msh2-/- mice

Project description:Expression profiling of FACS purified Lin-cKit+ cells from compound URE-/+::Msh2-/- mice with AML and control animals Analysis of gene expression in Lin-cKit+ cells from three URE-/+::Msh2-/- mice and four wild type controls

2015-09-07 | E-GEOD-65668 | biostudies-arrayexpress

Gene expression analysis of leukemia-initiating cells of compound URE-/+::Msh2-/- mice

Project description:Expression profiling of FACS purified Lin-cKit+ cells from compound URE-/+::Msh2-/- mice with AML and control animals

2015-09-07 | GSE65668 | GEO

Gene expression analysis of leukemia-initiating cells of preleukemic compound URE-/+::Msh2-/- mice

Project description:Expression profiling of FACS purified Lin-cKit+ cells from preleukemic compound URE-/+::Msh2-/- mice and control animals (two separate pools of 3 mice each) Analysis of gene expression in Lin-cKit+ cells from two URE-/+::Msh2-/- mice and wild type control animals (two pools of three animals each)

2015-09-07 | E-GEOD-65669 | biostudies-arrayexpress

Genomic maps of lnc-HLX-2-7 occupancy in human medulloblastoma cell line.

Project description:To gain more insights into the functional significance of lnc-HLX-2-7 in human medulloblastoma, genomic mapping of lnc-HLX-2-7 occupancy were analyzied by ChIRP DNA sequencing.

2023-11-01 | GSE232749 | GEO

Gene expression analysis of leukemia-initiating cells of preleukemic compound URE-/+::Msh2-/- mice

Project description:Expression profiling of FACS purified Lin-cKit+ cells from preleukemic compound URE-/+::Msh2-/- mice and control animals (two separate pools of 3 mice each)

2015-09-07 | GSE65669 | GEO

RNA sequencing analysis of intracranial D425 xenografts treated with ASO-CTRL and ASO-lnc-HLX 2-7

Project description:To gain more insights into the functional significance of lnc-HLX-2-7, gene expression profiles were measured using D425 (sh-CTRL), D425 (sh-HLX) and intracranial D425 xenografts treated with ASO-CTRL and ASO-lnc-HLX 2-7 by RNA sequencing.

2023-11-01 | GSE188746 | GEO

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