Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The role of LEDGF in transcription is exploited by HIV-1 to position integration [RNA]

ABSTRACT: HIV-1 integration occurs across actively transcribed genes due to the interaction of integrase with chromatin factor LEDGF. Although LEDGF was originally purified as a co-activator that stimulates promoters, this role is inconsistent with LEDGF-mediated integration across gene bodies and with data indicating LEDGF promotes transcriptional elongation. We found LEDGF is enriched primarily at transcription start sites (TSSs) of active promoters. Additional data shows MLL1 recruits LEDGF to promoters and LEDGF recruits RNA Pol II. Contrary to existing models, we find that integration into genes is independent of interactions between H3K36me3 and the PWWP domain of LEDGF. Instead, it is the level of LEDGF at TSSs that determines gene specificity of integration. These data support a dual role model of LEDGF where it is tethered to promoters by MLL1 and recruits RNA Pol II. Subsequently, LEDGF travels across the sequences of genes to effect HIV-1 integration.

ORGANISM(S): Homo sapiens

PROVIDER: GSE279646 | GEO | 2026/02/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The role of LEDGF in transcription is exploited by HIV-1 to position integration [ChIP-Seq]

Project description:HIV-1 integration occurs across actively transcribed genes due to the interaction of integrase with chromatin factor LEDGF. Although LEDGF was originally purified as a co-activator that stimulates promoters, this role is inconsistent with LEDGF-mediated integration across gene bodies and with data indicating LEDGF promotes transcriptional elongation. We found LEDGF is enriched primarily at transcription start sites (TSSs) of active promoters. Additional data shows MLL1 recruits LEDGF to promoters and LEDGF recruits RNA Pol II. Contrary to existing models, we find that integration into genes is independent of interactions between H3K36me3 and the PWWP domain of LEDGF. Instead, it is the level of LEDGF at TSSs that determines gene specificity of integration. These data support a dual role model of LEDGF where it is tethered to promoters by MLL1 and recruits RNA Pol II. Subsequently, LEDGF travels across the sequences of genes to effect HIV-1 integration.

2026-02-25 | GSE279687 | GEO

Identification of LEDGF-regulated genes.

2005-11-07 | GSE3485 | GEO

HIV-1 integration sites in the absence of host factors SET2D and MLL1.

Project description:HIV-1 integration sites were determined in wild-type HEK293T cells and cells lacking SET2D or MLL1. These experiments were performed to test the role of SET2D and MLL1 in LEDGF/p75-dependent HIV-1 integration site selection. Integration libraries were prepared by ligation-mediated PCR and sequenced on Illumina NextSeq 2000 for 150 bp paired-end sequencing.

2026-02-25 | GSE279615 | GEO

Identification of LEDGF-responsive genes in Jurkat cells

2007-04-13 | GSE7508 | GEO

RNAi of human Jurkat cells knocked down LEDGF transcript of PSIP1 gene

Project description:The LEDGF transcript from the PSIP1 gene was knocked down in Jurkat cells using RNAi technology. The resulting Jurkat-derived cell line (Jurkat-siJK2) was compared to a control cell line (wild type Jurkat) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Experiment Overall Design: 2 cell lines were used, Jurkat-siJK2 (experimental) and wild-type Jurkat (control). 3 independent cultures of each cell line were used for independent RNA extractions, labeling reactions and array hybridizations.

2008-06-15 | E-GEOD-7508 | biostudies-arrayexpress

RNAi knock down of LEDGF transcript from the PSIP1 gene in human 293T cells reveals a role for LEDFG/p75 in targeting HIV integration

Project description:The LEDGF transcript from the PSIP1 gene was knocked down using RNAi technology. The resulting 293T derived cell line (293TsiLL) was compared to a control cell line (293TsiScram) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Experiment Overall Design: 2 cell lines were used, 293TsiLL (experimental) and 293TsiScram (control). 4 independent cultures of each cell line were used for independent RNA extractions, labeling reactions and array hybridizations.

2007-10-27 | E-GEOD-3485 | biostudies-arrayexpress

The role of LEDGF in transcription is exploited by HIV-1 to position integration

Project description:The role of LEDGF in transcription is exploited by HIV-1 to position integration

| PRJNA1174084 | ENA

Role of LEDGF/p75 in MLL chemoresistance

Project description:The study has been focused on the characterization of the role of LEDGF/p75 in chemiresistance in pediatric leukemia Abstract: MLL is an aggressive subtype of leukemia with a poor prognosis that mostly affects pediatric patients. MLL-rearranged fusion proteins (MLLr) induce aberrant target gene expression resulting in leukemogenesis. MLL and its fusions are tethered to chromatin by LEDGF/p75, a transcriptional co-activator that specifically recognizes H3K36me2/3. LEDGF/p75 is ubiquitously expressed and associated with regulation of gene expression, autoimmune responses and HIV replication. LEDGF/p75 was proven to be essential for leukemogenesis in MLL. Apart from MLL, LEDGF/p75 has been linked to lung, breast and prostate cancer. Intriguingly, LEDGF/p75 interacts with Med-1, which co-localizes with BRD4. Both are known as co-activators of super-enhancers. Here, we describe LEDGF/p75-dependent chemoresistance of MLLr cell lines. Investigation of the underlying mechanism revealed a role of LEDGF/p75 in the cell cycle and in survival pathways and showed that LEDGF/p75 protects against apoptosis during chemotherapy. Remarkably, LEDGF/p75 levels also affected expression of BRD4 and Med1. Altogether, our data suggest a role of LEDGF/p75 in cancer survival, stem cell renewal, and activation of nuclear super enhancers.

2021-01-14 | GSE164751 | GEO

The role of LEDGF in transcription is exploited by HIV-1 to position integration [RNA]

Project description:The role of LEDGF in transcription is exploited by HIV-1 to position integration [RNA]

| PRJNA1173760 | ENA

The role of LEDGF in transcription is exploited by HIV-1 to position integration [ChIP-Seq]

Project description:The role of LEDGF in transcription is exploited by HIV-1 to position integration [ChIP-Seq]

| PRJNA1174089 | ENA