Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) analysis of ZNF148 in chondrocytes

ABSTRACT: Chromatin Immunoprecipitation (ChIP) was performed by fixing chondrocytes with formaldehyde, lysing them, and fragmenting chromatin via sonication. Specific antibodies of ZNF148 (Santa Cruz, sc-398148X, 200碌g/0.1ml) were used to immunoprecipitate protein-DNA complexes, followed by washing, reverse crosslinking and DNA purification for subsequent RT-qPCR or ChIP-seq analysis

ORGANISM(S): Homo sapiens

PROVIDER: GSE279679 | GEO | 2025/12/04

REPOSITORIES: GEO