Transcriptomics

Dataset Information

25

RNA-seq analysis of Tetrahymena thermophila


ABSTRACT: We present a comprehensive transcriptome of ciliate T. thermophila using the Illumina RNA-seq platform. The data was generated from the six mRNA samples of growth, starvation and conjugation of Tetrahymena. Despite an AT rich genome, there are about 124.6 million reads mapped to T. thermophila genome. Using these mapped reads, we have significantly improved the previous genome annotation and investigated the gene expression. Besides, our result also provided a comprehensive understanding of the alternative splicing in T. thermophila, and suggested the existence of the regulated unproductive splicing and translation (RUST) in the single-celled eukaryote. Overall design: RNA-seq for six samples of Tetrahymena growth, starvation and conjugation.

INSTRUMENT(S): Illumina Genome Analyzer (Tetrahymena thermophila)

ORGANISM(S): Tetrahymena thermophila  

SUBMITTER: Wei Miao  

PROVIDER: GSE27971 | GEO |

SECONDARY ACCESSION(S): PRJNA137763

REPOSITORIES: GEO

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