Gene expression profile of colorectal carcinoma HT29 PARP13 KO cells treated with fractionated ionizing radiation dose (5x2Gy) under 2D or 3D cell culture conditions

ABSTRACT: Radiotherapy is one of the most common treatments for cancer. The effects of IR are dose-dependent and different cell types have different sensitivities to IR. To reduce the damage of IR to the most sensitive cells of normal tissue, radiotherapy is administered as a fractionated dose treatment, delivering radiation in 2 Gy fractions every 24 hours, 5 times a week. However, during the course of therapy, intrinsic and acquired radioresistance of the tumor can lead to treatment failure. Therefore, there is a need for combination therapy including radiotherapy and targeted anticancer treatment. In the present study, we investigated global gene expression changes in colorectal cancer HT29 PARP13 KO cells after X-ray irradiation with a fractionated dose of 5 x 2 Gy. Agilent microarray technology was used to analyze gene expression levels in cells cultured under 2D and 3D culture conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE280331 | GEO | 2025/07/16

REPOSITORIES: GEO