Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional Integration of Autologous Tissue-Engineered Grafts in a Pre-Clinical Esophageal Defect Model

ABSTRACT: Congenital and acquired esophageal pathologies such as oesophageal atresia can leave large tissue deficits in patients were insufficient oesophagus exists to restore continuity. Currently, these require replacement by repurposing the stomach, colon or small bowel. The aim of this study was to develop a tissue engineered oesophagus as an alternative, produced using a clinically relevant timescale, and determine feasibility, safety of autologous transplantation of autologous grafts via a thoracic approach in a growing minipig model, together with assessment of function and integration.

ORGANISM(S): Sus scrofa

PROVIDER: GSE280737 | GEO | 2025/12/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene induction by low pH in oesophageal cells

Project description:Differential gene expression analysis of oesophageal cells stimulated with a low pH environment. Study designed to identify pathways involved in progression of gastro-oesophageal reflux disease through Barrett's oesophagus to adenocarcinoma. Identified many subsets of genes with involvement in pathogenesis. Keywords = GORD Keywords = Barrett's Oesophagus Keywords = Oesopageal Adenocarcinoma. Keywords: time-course

2005-10-04 | GSE2144 | GEO

Quantitative Shotgun Proteomics Unveils Candidate Novel Oesophageal Adenocarcinoma-Specific Proteins

Project description:Selective markers are needed for earlier diagnosis, optimal staging and treatment of oesophageal adenocarcinoma. This quantitative shotgun proteomic study used patient-matched fresh frozen tissue samples of OAC, normal oesophagus and normal stomach.

2017-03-31 | PXD004962 | Pride

Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels

Project description:Engineered exosomes reprogrammed Gli1+ cells to promote SMC reconstruction and inhibit calcification in TEVBs and autologous pathological vessels.

2023-08-08 | GSE227179 | GEO

RNA-seq of human Barrett's oesophagus and oesophageal adenocarcinoma samples

Project description:To investigate gene expression profiles of Barrett's oesophagus and oesophageal adenocarcinoma samples, we carried out RNA-seq to identify differentially expressed genes in each disease state.

2020-09-30 | E-MTAB-8584 | biostudies-arrayexpress

An in-depth analysis on vaginal wound healing post surgery: Transcriptomic effects of autologous micrografting versus acellular grafts

Project description:Understanding wound healing in vaginal tissue is crucial for improving female health outcomes, particularly in the context of surgeries like pelvic organ prolapse, post-radiation stricture formation and complications resulting from vaginal childbirth. Tissue-engineered grafts have proven successful for vaginal tissue repair, however, the mechanisms that stimulate tissue repair are unknown. This study aims to explore the transcriptomic signatures during tissue repair after using autologous micrografting versus acellular grafts in a rabbit model for surgical vaginal reconstruction. During a single surgical procedure, we created a graft for vaginal reconstruction consisting of autologous micrografts and supporting biomaterials. This graft was named "perioperative, layered, autologous, tissue expansion (PLATE)." We also produced acellular grafts that lacked autologous micrografts,. Our findings revealed distinct differences in gene expression patterns associated with wound healing, extracellular matrix organization, and smooth muscle regeneration between the two graft types. Notably, our findings revealed that micrografting showed an accelerated progression towards the final stages of wound healing, as evidenced by elevated matrix metalloproteinase (MMP) expression, particularly MMP-13. Additionally, Transforming Growth Factor Beta-1 (TGF-β1) emerged as an expected vaginal wound healing regulator. This study provides valuable insights into the molecular mechanisms of PLATE grafts and opens new avenues for the development of targeted therapies to improve wound healing in vaginal tissues.

2025-02-10 | GSE252016 | GEO

Microarray data of benzo[a]pyrene-treated WHCO1 vs EPC-2 cells

Project description:The aim of this project was to investigate the differential effects of benzo[a]pyrene (BaP) treatment on global gene transcription, comparing normal (EPC-2) and cancer (WHCO1) cell lines from the oesophagus, in order to further our understanding of the molecular mechanisms of BaP-induced development and maintenance of oesophageal squamous cell carcinoma.

2018-01-18 | GSE109316 | GEO

INTESTINAL FILTER FOR USE IN OESOPHAGEAL CANCER RESEARCH

Project description:This study utilise the examination of normal gastro-intestinal tissues to determine a tissue specific signal for use in deriving the intestinal signature of intestinal metaplasias of the oesophagus. Normal oesophageal, colonic and duodenal tissue biopsies were taken after informed consent and RNA was extracted following histological examination of adjacent tissues for normal aperaing mucosa.

2013-07-07 | GSE40220 | GEO

Transcription profiling of human oesophageal cells stimulated with a low pH environment

Project description:Differential gene expression analysis of oesophageal cells stimulated with a low pH environment. Study designed to identify pathways involved in progression of gastro-oesophageal reflux disease through Barrett's oesophagus to adenocarcinoma. Identified many subsets of genes with involvement in pathogenesis.<br><br>Missing data files: GSM38763.CEL (Time 0 mins rep 1), GSM38764.CEL (Time 0 mins rep 2), GSM38771.CEL (Time 240 mins rep 1), and GSM38772.CEL (Time 240 mins rep 2).

2007-08-25 | E-GEOD-2144 | biostudies-arrayexpress

The microRNA profile in neosquamous oesophageal mucosa following ablation of Barrett's oesophagus

Project description:mucosal biopsies were taken from patients at pre and post-argon plasma coagulation (APC) ablation of Barrett's oesophagus, and from healthy controls. Total RNA was extracted using Trizol. miRNA was reversed transcribed using a TaqMan® microRNA Reverse Transcription Kit (Life technologies, #4366596). miRNA profiling was performed with a high throughput TaqMan® OpenArray® Human microRNA panel (Life technologies, #4461104). The panel consisted of probes for 754 human miRNAs that are based on miRNA sequences derived from Sanger miRBase v14. Endoscopic surveillance remains the mainstay of cancer prevention in individuals with Barrett’s oesophagus, and definitive management by surgery or endoscopy is reserved for high grade dysplasia or cancer. There are several endoscopic treatments that are widely used for Barrett’s oesophagus, including argon plasma coagulation (APC). The normality of the regenerated neosquamous epithelium after ablation has been called into question. In the current study we profiled global microRNA expression in oesophageal mucosa before and after ablation of Barrett’s oesophagus using Argon plasma coagulation. Our aim was to investigate differences in microRNA expression between neosquamous and normal squamous tissues.

2017-09-14 | GSE94854 | GEO