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DDX20 knockdown effect on hepatocellular carcinoma cells

ABSTRACT: Analysis of DDX20 knockdown - hepatocellular carcinoma cells. The expression levels of genes driven by NF-kB and related with carcinogenesis, were significantly enhanced in DDX20-knockdown cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE28088 | GEO | 2012/07/15

SECONDARY ACCESSION(S): PRJNA139741

REPOSITORIES: GEO

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DDX20 knockdown effect on hepatocellular carcinoma cells

Project description:Analysis of DDX20 knockdown - hepatocellular carcinoma cells. The expression levels of genes driven by NF-kB and related with carcinogenesis, were significantly enhanced in DDX20-knockdown cells. One condition experiment, HepG2 vs. HepG2-DDX20 knockdown cells

2012-07-14 | E-GEOD-28088 | biostudies-arrayexpress

Epigenome analysis of normal and DDX20 knockdown HepG2 cells

Project description:MicroRNAs (miRNAs) are small RNAs that regulate the expression of specific target genes. While deregulated miRNA expression levels have been detected in many tumors, whether miRNA functional impairment is also involved in carcinogenesis remains unknown. We investigated whether deregulation of miRNA machinery components and subsequent functional impairment of miRNAs are involved in hepatocarcinogenesis. Among miRNA-containing ribonucleoprotein complex components, reduced expression of DDX20 was frequently observed in human hepatocellular carcinomas, in which enhanced NF-kB activity is closely linked to carcinogenesis. Because DDX20 normally suppresses NF-kB activity by preferentially regulating the function of the NF-kB suppressing miRNA-140, we hypothesized that impairment of miRNA-140 function may be involved in hepatocarcinogenesis. Dnmt1 was identified as a direct target of miRNA-140, and increased Dnmt1 expression in DDX20-deficient cells hypermethylated the promoters of metallothionein genes, resulting in decreased metallothionein expression leading to enhanced NF-kB activity and hepatocarcinogenesis. MiRNA-140 knockout mice were prone to hepatocarcinogenesis and showed phenomena similar to those of DDX20 deficiency, suggesting that miRNA-140 plays a central role in DDX20 deficiency–related pathogenesis. Conclusion: These results indicate that miRNA-140 acts as a liver tumor suppressor, and that impairment of miRNA-140 function due to a deficiency of DDX20, a miRNA machinery component, could lead to hepatocarcinogenesis. Genome wide DNA methylation profiling of control and DDX20-knockdown HepG2 cells. Bisulphite converted DNA from the 2 samples were hybridised to the Illumina HumanMethylation450 BeadChip.

2012-04-26 | E-GEOD-37633 | biostudies-arrayexpress

Epigenome analysis of normal and DDX20 knockdown HepG2 cells

2012-04-27 | GSE37633 | GEO

NFkB dependent gene expression in a Mdr2-KO hepatocellular carcinoma (HCC) mouse model using a IkB-Superrepressor

Project description:This study aims on the identification of the NF-kB dependent gene regulatory network during inflammation-associated liver carcinogenesis using the well-established Mdr2 knockout mouse model. We could identify 367 differentially expressed genes comparing expression profiles of tumor samples from Mdr2-KO mice to tumor samples derived from mice with an additional hepatocyte specific expression of an IkB-superrepressor. This IkB-superrepresser is undegradable upon ubiquitinylation initialized by Ikk dependent phosphorylation and therefore impedes NFkB activity. Keywords: NFkB, IkB-superrepressor, inflammation-associated liver carcinogenesis, Mdr2-KO mouse model, hepatocellular carcinoma, gene expression, microarray

2009-05-01 | GSE13599 | GEO

Stable knockdown of PLAGL2 in hepatocellular carcinoma cells

Project description:Stable knockdown of PLAGL2 in hepatocellular carcinoma cells

| PRJNA495569 | ENA

KCa3.1 depletion effect on hepatocellular carcinoma cell line Huh7

Project description:Analysis of Huh7 hepatocellular carcinoma (HCC) cells depleted for potassium channel KCa3.1, the intermediate conductance calcium-activated potassium channel. Result provide insight into the role of KCa3.1 in the carcinogenesis of HCC. We used microarrays to detail the gene expression differences between KCa3.1 knockdown and negative control in Huh7 HCC cells.

2018-06-23 | GSE116183 | GEO

Expression data from knockdown of Tyro3 in hepatocellular carcinoma

Project description:Expression data from knockdown of Tyro3 in hepatocellular carcinoma

| PRJNA489091 | ENA

RNA-seq reveals an abnormal transcriptome as a result of DDX20 loss in spermatogonia.

Project description:To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads.We then performed gene expression profiling analysis using data obtained from RNA-seq of THY1 + spermatogonia.

2023-10-01 | GSE217685 | GEO

Homo sapiens

Project description:DDX20 knockdown effect on hepatocellular carcinoma cells

| PRJNA139741 | ENA

Expression data regarding the effect of NPM1 knockdown on TNF-a induced gene expression in HeLa

Project description:NPM1 was reported to regulate the SOD2 gene expression through regulation of NF-kB. However, the effect of NPM1 on the NF-kB-dependent transcriptome has not been exmained. To examine the effect of NPM1 on NF-kB-dependent transcriptome. We used microarray to examine the effect of knockdown of NPM1 on the gene expression induced by TNF-a treatment which activates NF-kB pathway.

2017-02-01 | GSE81785 | GEO

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