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Developing exocrine glands harbour a distinct macrophage population that is maintained by ILC2 derived GM-CSF [CD45 scRNA-seq]

ABSTRACT: Granulocyte-macrophage colony-stimulating factor (GM-CSF) has a non-redundant role in the emergence and maintenance of alveolar macrophages (AMs). However, its role in steady-state myelopoiesis outside the lung is largely unexplored. We discovered a strong signal of GM-CSF production by type 2 innate lymphoid cells (ILC2s) in the developing salivary glands (SG). ILC2 derived GM-CSF fosters the development of a hitherto undescribed phagocyte subset, which we named adenophages. Detailed analysis focusing on phenotypic and transcriptional profiling revealed that adenophages represent a non-canonical macrophage subset displaying shared aspects of both, macrophages and dendritic cells. We found them to be homogenously distributed across the SG, but always forming a niche-triad with GM-CSF producing ILC2s and myoepithelial cells. Importantly, adenophages were present throughout all analyzed exocrine glands such as lacrimal glands and mammary glands, and were also identified in human SG, indicating a conserved role in exocrine glands across species.

ORGANISM(S): Mus musculus

PROVIDER: GSE280882 | GEO | 2025/10/28

REPOSITORIES: GEO

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Developing exocrine glands harbour a distinct macrophage population that is maintained by ILC2 derived GM-CSF [scRNA-seq]

Project description:Granulocyte-macrophage colony-stimulating factor (GM-CSF) has a non-redundant role in the emergence and maintenance of alveolar macrophages (AMs). However, its role in steady-state myelopoiesis outside the lung is largely unexplored. We discovered a strong signal of GM-CSF production by type 2 innate lymphoid cells (ILC2s) in the developing salivary glands (SG). ILC2 derived GM-CSF fosters the development of a hitherto undescribed phagocyte subset, which we named adenophages. Detailed analysis focusing on phenotypic and transcriptional profiling revealed that adenophages represent a non-canonical macrophage subset displaying shared aspects of both, macrophages and dendritic cells. We found them to be homogenously distributed across the SG, but always forming a niche-triad with GM-CSF producing ILC2s and myoepithelial cells. Importantly, adenophages were present throughout all analyzed exocrine glands such as lacrimal glands and mammary glands, and were also identified in human SG, indicating a conserved role in exocrine glands across species.

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