Dataset Information

Effects of interference and overexpression of NUDT3 on fat deposition in porcine subcutaneous adipocytes of pigs

ABSTRACT: The enforced expression of NUDT3 significantly suppressed adipogenesis and lipid accumulation in porcine subcutaneous adipocytes. In addition, exogenous expression of NUDT3 in adipose tissue significantly reduced fat expansion triggered by a high-fat diet in mice. Mechanistically, the DEGs affected by NUDT3 overexpression and interference were significantly enriched in the PI3K-Akt signaling pathway were revealed. Western blotting further confirmed that the phosphorylation of Akt was significantly increased by NUDT3 knockdown, and the levels of phosphorylated PI3K, Akt and mTOR were significantly decreased by the enforced expression of NUDT3 both ex vivo and in vivo.

ORGANISM(S): Sus scrofa

PROVIDER: GSE281004 | GEO | 2025/10/20

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:BackgroundChinese domestic pig breeds are reputed for pork quality, but their low ratio of lean-to-fat carcass weight decreases production efficiency. A better understanding of the genetic regulation network of subcutaneous fat tissue is necessary for the rational selection of Chinese domestic pig breeds. In the present study, subcutaneous adipocytes were isolated from Jiaxing Black pigs a Chinese indigenous pig breed with redundant subcutaneous fat deposition and Large White pigs a lean-type pig breed with relatively low subcutaneous fat deposition. The expression profiles of mRNAs and lncRNAs were compared by RNA-seq analysis to identify biomarkers correlated with the differences of subcutaneous fat deposition between the two breeds.ResultsA total of 1058 differentially expressed genes and 221 differentially expressed lncRNAs were identified in subcutaneous adipocytes between Jiaxing Black and Large White pigs, which included 275 up-regulated mRNAs, 783 down-regulated mRNAs, 118 up-regulated lncRNAs and 103 down-regulated lncRNAs. Gene Ontology and KEGG pathway enrichment analyses revealed that the differentially expressed genes and differentially expressed lncRNAs were mainly involved in the immune response, cell fate determination, PI3K-Akt signaling pathway and MAPK signaling pathway, which are known to be related to adipogenesis and lipid metabolism. The expression levels of differentially expressed genes and differentially expressed lncRNAs according to the RNA-seq data were verified by quantitative PCR, which showed 81.8% consistency. The differences in MAPK pathway activity between Jiaxing Black and Large White pigs was confirmed by western blot analysis, which revealed elevated p38 phosphorylation in Jiaxing Black pigs.ConclusionsThis study offers a detailed characterization of mRNAs and lncRNAs in fat- and lean-type pig breeds. The activity of the MAPK signaling pathway was found to be associated with subcutaneous adipogenesis. These results provide new targets for further investigation of the molecular mechanisms regulating subcutaneous fat deposition in pigs.

Dataset Information

Effects of interference and overexpression of NUDT3 on fat deposition in porcine subcutaneous adipocytes of pigs

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets