Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Monitoring ORF10's Impact on Host Transcriptome Using Doxycycline-Inducible A549 and 293T Cell Lines.

ABSTRACT: To monitor ORF10s effects on the host transcriptome, doxycycline-inducible cell lines were create in A549 and 293T(HEK) cells expressing ORF10. Because doxycycline itself can alter gene expression, we used doxycycline-inducible cell lines driving GFP as control. Empty vectors were used as well.

ORGANISM(S): Homo sapiens

PROVIDER: GSE281252 | GEO | 2024/12/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Monitoring ORF10's Impact on Host Transcriptome Using Doxycycline-Inducible A549 and 293T Cell Lines.

Project description:Monitoring ORF10's Impact on Host Transcriptome Using Doxycycline-Inducible A549 and 293T Cell Lines.

| PRJNA1182805 | ENA

Tumorigenic effects of TLX over-expression in HEK 293T cells

Project description:Transcriptomic profiling to investigate the effect of TLX overexpression with an inducible vector system in the HEK 293T cell line

2021-01-21 | GSE120893 | GEO

Small RNA deep sequencing of HEK 293T cells treated with an Adeno vector encoding the Vaccinia virus VP55 protein

Project description:To assess the efficacy of AdV-VP55 mediated degredation of host miRNAs. Small RNA profiles of HEK 293T cells treated with type 5 Adeno vectors expressing either GFP or GFP-VP55 for 24 hours

2016-08-03 | E-GEOD-74088 | biostudies-arrayexpress

RNA-seq of 293T cells expressing different RUNX1 splice isoforms

Project description:Biological relevance: RUNX1b is a transcription factor that is heavily implicated in leukemogenesis. In this study we identified that the RNA-binding protein hnRNP K binds to the RUNX1b transcript resulting in an alternatively spliced transcript that lacks Exon4. To determine the transcriptional consequences of this alternatively spliced variant, we performed RNA-Seq. Intent: To determine the effect of overexpression of RUNX1b or RUNX1b dEx4 Experimental Workflow: 293T cells were transduced with either empty vector, plasmids expressing RUNX1b full-length isoform or the RUNX1b dEx4 isoform. All plasmids used were doxycycline inducible. The cells were induced with doxycycline (0.4 mg/ml) for 72 hours, following which RNA was collected and sequenced. All experiments were done in triplicate.

2023-06-10 | E-MTAB-11870 | biostudies-arrayexpress

Over-expression of truncated FMRP with 295 amino acid residues: HEK293T cells

2015-05-08 | GSE68668 | GEO

Effect of depletion of C17orf80 on mtDNA transcription in HEK-293T cells

Project description:Effect of depletion of C17orf80 on mtDNA transcription in HEK-293T cells

2023-09-02 | GSE224121 | GEO

nsp1 acts on HEK-293T

Project description:nsp1 acts on HEK-293T

| PRJNA600748 | ENA

Over-expression of truncated FMRP with 295 amino acid residues: HEK293T cells

Project description:We used microarrays to detail the global gene expression in stably transfected HEK 293T cells of the over-expression of truncated FMRP containing 295 amino acid residues, which were compared with control (stably transfected HEK 293T cells of empty lentiviral vector (pLEX-MCS). Stably transfected HEK 293T cells of empty lentiviral vector (pLEX-MCS) and the over-expression of truncated FMRP were for RNA extraction and hybridization on Affymetrix microarrays.

2015-05-08 | E-GEOD-68668 | biostudies-arrayexpress

DNA methylation profiling for HEK-293T cells transfected with GFP and DMEΔ

Project description:Methylome data obtained from human embryonic kindey (HEK)-293T cells expressing a GFP (293T-GFP) or a truncated form of Arabidopsis DEMETER (DME) 5-methylcytosine (5mC) DNA glycosylase (293T-DMEΔ) analyzed on a Human Methylation 450K BeadChip platform (Illumina). These methylation array data revealed genome-wide DNA methylation patterns of the 293T-GFP cells (without direct 5mC excision activity) and 293T-DMEΔ cells (with artificially implemented direct 5mC excision activity).

2017-09-20 | GSE96864 | GEO

Enriched EWSR1 and EWS-FLI1 RNA-seq of HNRNPH1-silenced TC32 Ewing sarcoma and 293T human embryonic kidney cells.

Project description:Identification of EWSR1 and EWS-FLI1 transcript variants associated with the silencing of HNRNPH1 in TC32 Ewing sarcoma and 293T human embryonic kidney (HEK-293T) cells.

2019-09-20 | GSE119974 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data