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Expression data for 3D versus 2D cell culture

ABSTRACT: Ferroptosis is a regulated non-apoptotic cell death process characterized by iron-dependent lipid peroxidation. This process has recently emerged as a promising approach for cancer therapy. Some evidence indicates that GPX4 may be a useful target for drug development, yet factors that govern GPX4 inhibitor sensitivity in vivo are poorly understood. We find that pharmacological and genetic loss of GPX4 function was sufficient to induce ferroptosis in multiple adherent (“2D”) cancer cell cultures. However, reducing GPX4 protein levels did not affect tumor xenograft growth when these cells were implanted in mice. Furthermore, sensitivity to GPX4 inhibition was markedly reduced when cells were cultured as spheroids (“3D”). We utilized microarray to identify genome-wide expression changes in 3D versus 2D cell culture conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE281333 | GEO | 2025/08/18

REPOSITORIES: GEO

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