CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

ABSTRACT: We introduce CALIPERS, a Fluorescence Ubiquitin Cell Cycle Indicator enabling cell cycle-aware phenotyping based on multiplexed live-cell imaging. We validate CALIPERS in three- and four-color reporter lines (HaCaT) and human induced pluripotent stem cells (WTC-11), co-expressing genetically encoded structural and functional fluorescent sensors. To exemplify the broad applications of CALIPERS, we show cell cycle-aware phenotyping in live-cell imaging applications ranging from proliferation and migration to cardiac-specific maturation and drug testing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE281528 | GEO | 2026/06/25

REPOSITORIES: GEO