Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exploring the Transformative Effects of Calorie Restriction on the Lacrimal Gland in Adult Mice

ABSTRACT: Dry eye disease affects millions worldwide and can result from age-related lacrimal gland (LG) dysfunction, which correlates with a decline in LG secretory cell function and chronic inflammation. This study investigates the potential of calorie restriction (CR) to maintain LG and ocular surface health. Female C57BL/6J mice were subjected to 40% CR for 4 months (start age 6/7 until 10/11M) and compared to mice receiving food ad libitum (AL). LGs bulk RNA sequencing was performed, and fastq files were uploaded on ROSALIND® for processing. Differentially Expressed Genes (DEGs) were analyzed in Metascape to identify the biological pathways significantly modified by CR, and findings were validated. RNA sequencing of LGs subjected to CR compared to ad libitum revealed 589 significant DEGs, with 259 downregulated and 330 upregulatedPathways enriched in upregulated DEGs suggested increase in secretory functions and lipid metabolism, while pathways enriched in downregulated DEGs are related to immune cell activation, the adaptive immune system, extracellular matrix remodeling, and metalloproteinases. These changes were validated by individual qPCR. CR also decreased the age-related dry eye phenotype compared to the ad libitum group. Our results suggest that CR can maintain the health of the LG and ocular surface by reducing inflammation and supporting the LG's secretory function.

ORGANISM(S): Mus musculus

PROVIDER: GSE281747 | GEO | 2025/12/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Exploring the Transformative Effects of Calorie Restriction on the Lacrimal Gland in Adult Mice

Project description:Advanced age is one of the most recognizable risk factors for dry eye. Dry eye disease affects millions worldwide and can result from age-related lacrimal gland dysfunction, which correlates with a decline in lacrimal gland secretory cell function and chronic inflammation. This study investigated the potential of calorie restriction to maintain LG and ocular surface health. Adult female C57BL/6J mice were subjected to a 40% calorie restriction for four months, starting at 6–7 months and continuing until 10–11 months. These mice were compared to controls fed ad libitum. Bulk RNA sequencing of lacrimal glands, conjunctiva and cornea subjected to calorie restriction compared to ad libitum revealed significant differentially expressed genes (DEGs). Pathways enriched in the upregulated DEGs indicate enhanced circadian rhythm, secretory functions and lipid metabolism. These findings were confirmed using individual qPCR reactions and western blotting. In contrast, pathways enriched in the downregulated DEGs were associated with immune cell activation, adaptive immune responses, extracellular matrix remodeling, and metalloproteinase activity. Histological sections of calorie-restricted lacrimal glands revealed reduced mononuclear cell infiltration and fewer positive cells for CD4, CD19, and MHC II compared to libitum lacrimal glands. Calorie restriction also prevented age-related corneal barrier dysfunction and mitigated age-related conjunctival goblet cell loss, hallmarks of dry eye disease. These findings suggest that calorie restriction supports lacrimal gland and ocular surface health by reducing inflammation and extracellular matrix remodeling and by enhancing the lacrimal glands secretory function.

2025-06-17 | GSE298317 | GEO

Transcription profiling of rat lacrimal gland samples from normal, sham and operated animals

Project description:Previous studies showed that loss of muscarinic parasympathetic input to the lacrimal gland (LG) leads to a dramatic reduction in tear secretion and profound changes to LG structure. In this study, we used DNA microarrays to examine the regulation of the gene expression of the genes for secretory function and organization of the LG. Long-Evans rats anesthetized with a mixture of ketamine/xylazine (80:10 mg/kg) underwent unilateral sectioning of the greater superficial petrosal nerve, the input to the pterygopalatine ganglion. After 7 days, tear secretion was measured, the animals were killed, and structural changes in the LG were examined by light microscopy. Total RNA from control and experimental LGs (n = 5) was used for DNA microarray analysis employing the U34A GeneChip. Control rat lacrimal gland samples representing a normal unoperated, a sham, and paired contralateral controls. Experimental LG samples representing paired and unpaired contralateral controls. Paired contralateral control LGs represent LG from the same animal, one side was operated and the other side unoperated.

2007-10-12 | E-GEOD-844 | biostudies-arrayexpress

Lacrimal Gland from Sjögren's disease animal model

Project description:The lacrimal gland (LG) is the major source of aqueous tears, and insufficient LG secretion leads to aqueous-deficient dry eye (ADDE) disease. To provide a foundational description of LG's protein expression patterns, we prepared protein extracts of LGs from a wild-type and an ADDE mouse model and analyzed the proteome by quantitative mass spectrometry.

2025-04-18 | PXD060937 | Pride

Age Map, Spinal Cord (B)

Project description:To assess the transcriptional changes in different brain regions and spinal cord associated with aging and either a caloric restricted (CR) or ad libitum (AL) diet. cDNA microarray and quantitative PCR analyses were used to examine the transcriptomes of various neuronal tissues in young, middle aged and old mice. Mice of both genders were examined as well as the effects of their placement on either caloric restricted (CR) or ad libitum (AL) diets. To assess the transcriptional changes in different brain regions and spinal cord associated with aging and either a caloric restricted (CR) or ad libitum (AL) diet. cDNA microarray and quantitative PCR analyses were used to examine the transcriptomes of various neuronal tissues in young, middle aged and old mice. Mice of both genders were examined as well as the effects of their placement on either caloric restricted (CR) or ad libitum (AL) diets. Keywords: age-, gender-, and diet- comparison

2007-12-28 | GSE8418 | GEO

Age Map, Striatum (B)

2007-12-28 | GSE8419 | GEO

Age Map, Cerebral Cortex (B)

2007-12-28 | GSE8415 | GEO

Age Map, Cerebellum (B)

2007-12-28 | GSE8414 | GEO

Age Map, Cerebellum (A)

2009-06-22 | GSE8409 | GEO

Age Map, Hippocampus (B)

2007-12-28 | GSE8417 | GEO

Age Map, Striatum (A)