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Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE282040 | GEO | 2025/04/11

REPOSITORIES: GEO

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Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells [smallRNA-seq]

Project description:Transposable elements (TEs) pose a threat to genome integrity, and the piRNA pathway in animal gonads plays a crucial role in silencing TE activity. While the transcriptional regulation of piRNA pathway components in germline cells is well understood, the mechanisms orchestrating the transcriptional program of the somatic piRNA pathway in surrounding somatic cells remains poorly defined. Here, we demonstrate that Traffic Jam (Tj), an orthologue of a large Maf transcription factor in mammals, is a master regulator of the somatic piRNA pathway playing a crucial role in maintaining TE silencing and genomic integrity in somatic tissues . We show that Tj directly binds to the promoters of piRNA factors such as fs(1)Yb, nxf2, panx, and armi, as well as the flamenco piRNA cluster, a major locus for TE silencing in somatic cells. Knockdown of Tj in somatic follicle cells results in significant downregulation of these piRNA factors, a complete loss of flam expression, and derepression of TEs. We have identified an enhancer downstream of the flam promoter, including TJ binding sites, essential for high and specific flam expression in ovarian follicle cells in adult ovaries. This work uncovers a previously unappreciated layer of transcriptional regulation oc the piRNA pathway and we propose that Tj evolved to orchestrate the somatic piRNA pathway expression, ensuring efficient TE silencing in somatic cells that support germ cells, thereby preventing instability from being transmitted to the offspring.

2025-04-11 | GSE282039 | GEO

Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells [RNA-seq]

2025-04-11 | GSE275646 | GEO

Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells

Project description:Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells

| PRJNA1186699 | ENA

Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells [RNA-seq]

Project description:Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells [RNA-seq]

| PRJNA1152487 | ENA

Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells [smallRNA-seq]

Project description:Transcriptional factor Tj is the master regulator of the somatic piRNA pathway in the Drosophila ovarian follicle cells [smallRNA-seq]

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Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway.

Project description: Not available

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Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila.

Project description: Not available

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An mRNA/3' UTR-directed primary piRNA pathway in Drosophila ovarian somatic cells

Project description:Piwi-interacting RNAs (piRNAs) are ~24-30 nucleotide regulatory RNAs that are abundantly expressed in gonads. The most well-understood piRNAs mediate post-transcriptional defense against transposable elements (TEs), and derive from sense or antisense strands as a consequence of "ping-pong" amplification of complementary sequences of active TEs and piRNA master control transcripts. Another class of piRNAs, such as those expressed in pachytene testis, derive from large intergenic clusters that are strictly single-stranded. Here, we report a third substrate that generates abundant primary piRNAs. In somatic follicle cells of Drosophila ovaries, we cloned >1 million piRNAs from thousands of messenger RNAs, and these were quite preferentially derived from 3' untranslated regions. This segregation implies a competition between the translation machinery and primary piRNA biogenesis machinery for mRNA access.

2010-01-04 | GSE15825 | GEO

An mRNA/3' UTR-directed primary piRNA pathway in Drosophila ovarian somatic cells

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A somatic piRNA pathway in the Drosophila fat body ensures metabolic homeostasis and normal lifespan.

Project description: Not available

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