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Glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling

ABSTRACT: Metabolic pathways can influence cell fate decisions, but their regulative role during embryonic development remains poorly understood. Here, we demonstrate a crucial role of glycolytic activity in regulating signalling pathways involved in mesoderm and endoderm specification. Using an mESC-based in vitro model for gastrulation, we found that glycolysis inhibition increases ectodermal cell fates at the expense of mesodermal and endodermal lineages. We used single cell RNA sequencing (scRNA-seq) to analyze the mechanistic insight into the nutritional regulation of cell fate decision making.

ORGANISM(S): Mus musculus

PROVIDER: GSE282050 | GEO | 2025/03/14

REPOSITORIES: GEO

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