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Identifying the signaling cascades and regulatory mechanisms that control osmotic stress response

ABSTRACT: We performed knockout and wild type expression experiments in yeast grown in soribtol to identify genes invovled in controling osmotic stress response.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE28213 | GEO | 2012/07/10

SECONDARY ACCESSION(S): PRJNA139661

REPOSITORIES: GEO

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Identifying the signaling cascades and regulatory mechanisms that control osmotic stress response

Project description:We performed knockout and wild type expression experiments in yeast grown in soribtol to identify genes invovled in controling osmotic stress response. Soribitol experiments were performed by growing to logarithmic phase in synthetic complete (SC) medium and then washing and resuspending in SC medium with 1M sorbitol for 30 minutes. Two replicates were performed per knockout strain and compared with two or four control replicates depending on the platform.

2012-07-09 | E-GEOD-28213 | biostudies-arrayexpress

Osmotic stress on yeast S. cerevisiae

Project description:mRNA amount (RA) and Transcription rate (TR) analysis of W303-1a (wt) and hog1 mutant yeast strains growing in exponential phase in YPD subjected to osmotic stress This SuperSeries is composed of the following subset Series: GSE13096: Transcription rate analysis of wild type strain subjected to osmotic stress GSE13097: mRNA amount analysis of wild type strain subjected to osmotic stress GSE13098: Transcription rate analysis of W303 hog1 mutant strain subjected to osmotic stress GSE13099: mRNA amount analysis of W303 hog1 mutant strain subjected to osmotic stress Refer to individual Series Transcriptomic and transcription rate analysis by means of GRO of three independent replicates the yeast strain growing in exponential phase. Each time point replicate has been hybridized on a different macroarray (F11-F24). A single DNA genomic hybridization from the same labeling reaction was done on the same microarrays for normalization.

2010-06-19 | E-GEOD-13100 | biostudies-arrayexpress

Arabidopsis germination and two week growth on ionic vs osmotic stress

Project description:Arabidopsis Col-0 seeds were germinated and grown for two weeks on Arabidopsis thaliana salt media (ATS, control) or ATS media supplemented 50, 75, 100 or 125 mM NaCl that imposes both an ionic and osmotic stress; or ATS media supplemented with iso-osmolar concentrations of sorbitol (100, 150, 200 or 250 mM) that imposes only an osmotic stress. The aim of the study was to identify genes involved in plant growth and adaptation to ionic stress compared to genes involved in growth and adaptation to osmotic stress conditions. To do this we identified lists of genes that are differentially expressed in plants grown in NaCl (A) and lists of genes differentially expressed in plants grown in sorbitol (B). We then compared these lists to find ionic/salt-specific genes that are only expressed in plants grown in NaCl and not in plants grown in sorbitol; and osmotic genes that are expressed both in plants grown in NaCl and in plants grown in sorbitol. Associated publication: Cackett et al. (2022) Salt-specific gene expression reveals elevated auxin levels in Arabidopsis thaliana plants grown under saline conditions, DOI: 10.3389/fpls.2022.804716

2022-01-16 | GSE193762 | GEO

Osmotic stress on yeast S. cerevisiae

2009-04-23 | GSE13100 | GEO

mRNA amount analysis of wild type strain subjected to osmotic stress

Project description:mRNA amount (RA) analysis of W303-1a yeast strain growing in exponential phase in YPD subjected to osmotic stress Keywords: time course

2009-04-23 | GSE13097 | GEO

Transcription rate analysis of wild type strain subjected to osmotic stress

Project description:Transcription rate (TR) analysis of W303-1a yeast strain growing in exponential phase in YPD subjected to osmotic stress Keywords: time course

2009-04-23 | GSE13096 | GEO

mRNA amount analysis of W303 hog1 mutant strain subjected to osmotic stress

Project description:mRNA amount (RA) analysis of W303 hog1 mutant yeast strain growing in exponential phase in YPD subjected to osmotic stress Keywords: Time course

2009-04-23 | GSE13099 | GEO

Transcription rate analysis of W303 hog1 mutant strain subjected to osmotic stress

Project description:Transcription rate (TR) analysis of W303 hog1 mutant yeast strain growing in exponential phase in YPD subjected to osmotic stress Keywords: Time course

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SWATH Analysis of Yeast Proteome over Time in Response to Osmotic Stress

Project description:SWATH analysis of Yeast Proteome over time in response to Osmotic Stress. We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode. Proteins were quantified by the targeted extraction and signal integration from the SWATH-MS datasets of peak groups representing proteotypic peptides for specific yeast proteins.

2017-11-03 | MSV000081677 | MassIVE

Comparative proteomic analysis of Arabidopsis plants treated with osmotic stress

Project description:To evaluate the differences in the acclimation of gcd1 gcd3 and WT plants to osmotic stress, we conducted an LC-MS/MS-based label-free quantitative proteomics analysis of WT and gcd1 gcd3 seedlings grown under mock conditions or osmotic stress imposed by 300 mM mannitol treatment. Comparative proteomic analysis of plants treated with osmotic stress reveals the basis of the underlying acclimation mechanism.

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