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Spontaneous Immortalization of Bovine Fibroblasts through Extended In Vitro Culture

ABSTRACT: Spontaneous immortalization in large animals like cattle is rare and has previously required genetic manipulation of TP53 or TERT. Here, we demonstrate the spontaneous immortalization of fibroblast lines from Holstein and Simmental cows over 500 days of continuous culture, corresponding to 240 population doublings. Initial senescence was observed at population doubling 60, marked by telomere shortening, γH2AX foci, and an active SASP profile. After 400 days, stable immortalized lines with a doubling time of 45 hours emerged. Transcriptomic and metabolic analyses revealed a reversal of senescence-associated apoptotic processes and mitochondrial stress, with no change in telomerase or p53 activity. Our findings suggest bovine fibroblast immortalization can occur through alternative pathways, with applications in cultivated meat production and aging research.

ORGANISM(S): Bos taurus

PROVIDER: GSE282140 | GEO | 2025/09/08

REPOSITORIES: GEO

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