Rhinolophus sinicus limb embryonic development single cell transcriptome
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus Rhinolophus sinicus
PROVIDER: GSE282269 | GEO | 2026/04/15
REPOSITORIES: GEO
Similar Datasets
Project description:The bat offers an alternative paradigm to the standard mouse and chick model of limb development as it has extremely divergent forelimbs (long digits supporting a wing) and hindlimbs (short digits and claws) due the distinct requirements of both aerial and terrestrial locomotion. We used a cross-species microarray approach to identify differentially expressed (DE) genes between the bat (Minniopterus natalensis) forelimb and hindlimb autopods at Carollia developmental stages (CS) 16 and CS17, and between the bat (CS17) and mouse (E13.5) forelimb autopods. Several DE genes were identified, including two homeobox genes, Meis2, a proximal limb-patterning gene, and Hoxd11, a gene involved in digit elongation. Both genes are significantly over-expressed in the developing bat forelimb as compared to the hindlimb and equivalently staged mouse forelimbs.
2014-02-15 | GSE51042 | GEO
Project description:The bat offers an alternative paradigm to the standard mouse and chick model of limb development as it has extremely divergent forelimbs (long digits supporting a wing) and hindlimbs (short digits and claws) due the distinct requirements of both aerial and terrestrial locomotion. We used a cross-species microarray approach to identify differentially expressed (DE) genes between the bat (Minniopterus natalensis) forelimb and hindlimb autopods at Carollia developmental stages (CS) 16 and CS17, and between the bat (CS17) and mouse (E13.5) forelimb autopods. Several DE genes were identified, including two homeobox genes, Meis2, a proximal limb-patterning gene, and Hoxd11, a gene involved in digit elongation. Both genes are significantly over-expressed in the developing bat forelimb as compared to the hindlimb and equivalently staged mouse forelimbs. A reference design was used in this microarray experiment. A pool of left and right mouse forelimb autopods from 24 embryos was used as the reference sample. This sample was directly compared to individual CS16 and CS17 bat fore- and hindlimbs (left and right of one individual pooled) that were classified as the test conditions. Four experimental sessions were performed using an independently amplified mouse reference pool and 4 biological repeats for the bat limbs. These samples were co-hybridised to OPERON Mouse OpArray (ver. 4.0) spotted oligonucleotide slides to perform a competitive Cross-Species Hybridisation experiment. The bat aRNA (test) samples were labelled with Cy3 dye (green signal), the mouse aRNA (reference) sample was labelled with Cy5 dye (red signal).
2014-02-15 | E-GEOD-51042 | biostudies-arrayexpress
Project description:As the only truly flying mammals, bats use their unique wing formed from elongated digits connected by membranes to power their flight. The forelimb of bats consists of four elongated digits (digits II-V) and one shorter digit (digit I) that is morphologically similar to the hindlimb digits. Elongation of bat forelimb digits is thought to results from changes in the temporal and spatial expression of a number of developmental genes. As a result, comparing gene expression profiles between short and elongated digit morphologies of the fore- and hindlimbs may elucidate the molecular mechanisms underlying digit elongation in bats. Here, we performed a large-scale analysis of gene expression of forelimb digit I, forelimb digits II-V, and all five hindlimb digits in Myotis ricketti using digital gene expression tag profiling approach. Results of this study not only implicate several developmental genes as robust candidates underlying digit elongation in bats, but also provide a better understanding of the genes involved in autopodial development in general. A large-scale analysis of gene expression of 3 different parts of autopods in Myotis ricketti using digital gene expression tag profiling approach.
2010-12-06 | E-GEOD-20038 | biostudies-arrayexpress
Project description:As the only truly flying mammals, bats use their unique wing formed from elongated digits connected by membranes to power their flight. The forelimb of bats consists of four elongated digits (digits II-V) and one shorter digit (digit I) that is morphologically similar to the hindlimb digits. Elongation of bat forelimb digits is thought to results from changes in the temporal and spatial expression of a number of developmental genes. As a result, comparing gene expression profiles between short and elongated digit morphologies of the fore- and hindlimbs may elucidate the molecular mechanisms underlying digit elongation in bats. Here, we performed a large-scale analysis of gene expression of forelimb digit I, forelimb digits II-V, and all five hindlimb digits in Myotis ricketti using digital gene expression tag profiling approach. Results of this study not only implicate several developmental genes as robust candidates underlying digit elongation in bats, but also provide a better understanding of the genes involved in autopodial development in general.
2010-12-06 | GSE20038 | GEO
Project description:Bats (order Chiroptera) are the only mammals capable of self-powered flight. Unlike in humans and mice, digits II-V in the bat forelimb are not separated but connected by the chiropatagium, an elastic membrane that forms the wing. The molecular underpinnings of these morphological differences, one of the most striking adaptations in mammalian evolution, remain obscure. Here, we use a suite of omics tools and single-cell analyses to compare the development of fore- and hindlimbs, in bat and mouse. We demonstrate a clear conservation of cell states and processes between species, even at the level of apoptosis, a mechanism that removes the tissue between the digits. To trace down the cellular origin of the persistent interdigital cells in bats, we micro-dissected the embryonic chiropatagium and performed single-cell transcriptomics. We found that the chiropatagium cells develop from fibroblasts populations, and independently from the apoptosis-related retinoic acid signaling cells of the interdigital mesenchyme. These cells from the distal part of the limb repurpose a gene program otherwise only present in proximal cells. Epigenomic and gene network analyses revealed two transcription factors, MEIS2 and TBX3, among the most prominent regulators of this gene program. Transgenic ectopic expression of MEIS2 and TBX3 in interdigital cells of mice resulted in the activation of genes associated with bat wing processes. Major morphological changes in these mutants include an increase in autopod volume, extracellular matrix and the partial retention of interdigital tissue with fusion (syndactyly) of digits. Our results elucidate fundamental molecular mechanisms of wing formation in bats. More generally, we illustrate that the repurposing of existing developmental programs is an evolutionary molecular mechanism to generate morphological novelties.
2025-07-16 | GSE275855 | GEO
Project description:Bats (order Chiroptera) are the only mammals capable of self-powered flight. Unlike in humans and mice, digits II-V in the bat forelimb are not separated but connected by the chiropatagium, an elastic membrane that forms the wing. The molecular underpinnings of these morphological differences, one of the most striking adaptations in mammalian evolution, remain obscure. Here, we use a suite of omics tools and single-cell analyses to compare the development of fore- and hindlimbs, in bat and mouse. We demonstrate a clear conservation of cell states and processes between species, even at the level of apoptosis, a mechanism that removes the tissue between the digits. To trace down the cellular origin of the persistent interdigital cells in bats, we micro-dissected the embryonic chiropatagium and performed single-cell transcriptomics. We found that the chiropatagium cells develop from fibroblasts populations, and independently from the apoptosis-related retinoic acid signaling cells of the interdigital mesenchyme. These cells from the distal part of the limb repurpose a gene program otherwise only present in proximal cells. Epigenomic and gene network analyses revealed two transcription factors, MEIS2 and TBX3, among the most prominent regulators of this gene program. Transgenic ectopic expression of MEIS2 and TBX3 in interdigital cells of mice resulted in the activation of genes associated with bat wing processes. Major morphological changes in these mutants include an increase in autopod volume, extracellular matrix and the partial retention of interdigital tissue with fusion (syndactyly) of digits. Our results elucidate fundamental molecular mechanisms of wing formation in bats. More generally, we illustrate that the repurposing of existing developmental programs is an evolutionary molecular mechanism to generate morphological novelties.
2025-07-16 | GSE275854 | GEO
Project description:Bats (order Chiroptera) are the only mammals capable of self-powered flight. Unlike in humans and mice, digits II-V in the bat forelimb are not separated but connected by the chiropatagium, an elastic membrane that forms the wing. The molecular underpinnings of these morphological differences, one of the most striking adaptations in mammalian evolution, remain obscure. Here, we use a suite of omics tools and single-cell analyses to compare the development of fore- and hindlimbs, in bat and mouse. We demonstrate a clear conservation of cell states and processes between species, even at the level of apoptosis, a mechanism that removes the tissue between the digits. To trace down the cellular origin of the persistent interdigital cells in bats, we micro-dissected the embryonic chiropatagium and performed single-cell transcriptomics. We found that the chiropatagium cells develop from fibroblasts populations, and independently from the apoptosis-related retinoic acid signaling cells of the interdigital mesenchyme. These cells from the distal part of the limb repurpose a gene program otherwise only present in proximal cells. Epigenomic and gene network analyses revealed two transcription factors, MEIS2 and TBX3, among the most prominent regulators of this gene program. Transgenic ectopic expression of MEIS2 and TBX3 in interdigital cells of mice resulted in the activation of genes associated with bat wing processes. Major morphological changes in these mutants include an increase in autopod volume, extracellular matrix and the partial retention of interdigital tissue with fusion (syndactyly) of digits. Our results elucidate fundamental molecular mechanisms of wing formation in bats. More generally, we illustrate that the repurposing of existing developmental programs is an evolutionary molecular mechanism to generate morphological novelties.
2025-07-16 | GSE275853 | GEO
Project description:Bats (order Chiroptera) are the only mammals capable of self-powered flight. Unlike in humans and mice, digits II-V in the bat forelimb are not separated but connected by the chiropatagium, an elastic membrane that forms the wing. The molecular underpinnings of these morphological differences, one of the most striking adaptations in mammalian evolution, remain obscure. Here, we use a suite of omics tools and single-cell analyses to compare the development of fore- and hindlimbs, in bat and mouse. We demonstrate a clear conservation of cell states and processes between species, even at the level of apoptosis, a mechanism that removes the tissue between the digits. To trace down the cellular origin of the persistent interdigital cells in bats, we micro-dissected the embryonic chiropatagium and performed single-cell transcriptomics. We found that the chiropatagium cells develop from fibroblasts populations, and independently from the apoptosis-related retinoic acid signaling cells of the interdigital mesenchyme. These cells from the distal part of the limb repurpose a gene program otherwise only present in proximal cells. Epigenomic and gene network analyses revealed two transcription factors, MEIS2 and TBX3, among the most prominent regulators of this gene program. Transgenic ectopic expression of MEIS2 and TBX3 in interdigital cells of mice resulted in the activation of genes associated with bat wing processes. Major morphological changes in these mutants include an increase in autopod volume, extracellular matrix and the partial retention of interdigital tissue with fusion (syndactyly) of digits. Our results elucidate fundamental molecular mechanisms of wing formation in bats. More generally, we illustrate that the repurposing of existing developmental programs is an evolutionary molecular mechanism to generate morphological novelties.
2025-07-16 | GSE275851 | GEO
Project description:Bats (order Chiroptera) are the only mammals capable of self-powered flight. Unlike in humans and mice, digits II-V in the bat forelimb are not separated but connected by the chiropatagium, an elastic membrane that forms the wing. The molecular underpinnings of these morphological differences, one of the most striking adaptations in mammalian evolution, remain obscure. Here, we use a suite of omics tools and single-cell analyses to compare the development of fore- and hindlimbs, in bat and mouse. We demonstrate a clear conservation of cell states and processes between species, even at the level of apoptosis, a mechanism that removes the tissue between the digits. To trace down the cellular origin of the persistent interdigital cells in bats, we micro-dissected the embryonic chiropatagium and performed single-cell transcriptomics. We found that the chiropatagium cells develop from fibroblasts populations, and independently from the apoptosis-related retinoic acid signaling cells of the interdigital mesenchyme. These cells from the distal part of the limb repurpose a gene program otherwise only present in proximal cells. Epigenomic and gene network analyses revealed two transcription factors, MEIS2 and TBX3, among the most prominent regulators of this gene program. Transgenic ectopic expression of MEIS2 and TBX3 in interdigital cells of mice resulted in the activation of genes associated with bat wing processes. Major morphological changes in these mutants include an increase in autopod volume, extracellular matrix and the partial retention of interdigital tissue with fusion (syndactyly) of digits. Our results elucidate fundamental molecular mechanisms of wing formation in bats. More generally, we illustrate that the repurposing of existing developmental programs is an evolutionary molecular mechanism to generate morphological novelties.
2025-07-16 | GSE275848 | GEO
Project description:In vitro models allow for the study of developmental processes outside of the embryo. To gain access to the cells mediating digit and joint development, we identified a unique property of undifferentiated mesenchyme isolated from the distal early autopod to autonomously re-assemble forming multiple autopod structures including: digits, interdigital tissues, joints, muscles and tendons. Single cell transcriptomic analysis of these developing structures revealed distinct cell clusters that express canonical markers of distal limb development including: Col2a1, Col10a1, and Sp7 (phalanx formation) Thbs2 and Col1a1, (perichondrium), Gdf5, Wnt5a, and Jun (joint interzone), Aldh1a2 and Msx1 (interdigital tissues), Myod1 (muscle progenitors), Prg4 (articular perichondrium/articular cartilage), and Scx and Tnmd (tenocytes/tendons). Analysis of the gene expression patterns for these signature genes indicates that developmental timing and tissue-specific localization were also recapitulated in a manner similar to the initiation and maturation of the developing murine autopod. Finally, the in vitro digit system also recapitulates congenital malformations associated with genetic mutations as in vitro cultures of Hoxa13 mutant mesenchyme produced defects present in Hoxa13 mutant autopods including digit fusions, reduced phalangeal segment numbers, and poor mesenchymal condensation. These findings demonstrate the robustness of the in vitro digit system to recapitulate digit and joint development. As an in vitro model of murine digit and joint development, this innovative system will provide access to developing limb tissues facilitating studies to discern how digit and articular joint formation is initiated and how undifferentiated mesenchyme is patterned to establish individual digit morphologies. The in vitro digit system also provides a platform to rapidly evaluate treatments aimed at stimulating the repair or regeneration of mammalian digits impacted by congenital malformation, injury, or disease.
2023-02-23 | GSE221883 | GEO