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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE282280 | GEO | 2025/08/08

REPOSITORIES: GEO

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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Postnatal Retrotransposon expression in spermatogenesis]

Project description:Silencing of young retrotransposons by Cytosine DNA methylation is essential for spermatogenesis. Failure to methylate retrotransposon promoters leads to their reactivation, meiotic catastrophe and infertility. How transposons become reactivated and why their reactivation follows spermatogenic developmental patterns remains unclear. Here, we show that specific retrotransposon families exhibit distinct expression patterns and chromatin landscapes throughout spermatogenesis when DNA methylation is absent from their promoters. We find a strong correlation between the loss of bivalent H3K4me3-H3K27me3 chromatin modifications and the transition from low retrotransposon expression in spermatogonia to reactivation in meiotic spermatocytes. Using a combination of DNA pulldowns, mass spectrometry and chromatin profiling by CUT&Tag, we identify the DNA methylation-sensitive transcription factor NRF1 as a potential regulator of unmethylated retrotransposons in spermatogenesis. Germline conditional ablation of Nrf1 reduced the upregulation of IAPs in the absence of DNA methylation and rescued the accumulation of IAP-derived Pol protein to wild type germ cell levels. Our study demonstrates that a combination of chromatin modifications and a DNA methylation-sensitive TF regulates young retrotransposons upon loss of repressive DNA methylation in germ cells, suggesting these interactions may be a core strategy used by retrotransposons to proliferate in the germline after evading silencing by DNA methylation.

2025-08-08 | GSE282278 | GEO

DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Retrotransposon expression in conditional NRF1 mutant testes]

2025-08-08 | GSE282279 | GEO

DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Profiling of identified candidate TFs in spermatogenesis]

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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation.

Project description:DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation.

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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Chromatin landscape across spermatogenesis in wild type and Dnmt3C mutants]

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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Postnatal Retrotransposon expression in spermatogenesis]

Project description:DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Postnatal Retrotransposon expression in spermatogenesis]

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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Profiling of identified candidate TFs in spermatogenesis]

Project description:DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Profiling of identified candidate TFs in spermatogenesis]

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DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Retrotransposon expression in conditional NRF1 mutant testes]

Project description:DNA methylation-sensitive transcription factors and bivalency orchestrate transposon expression in the absence of DNA methylation [Retrotransposon expression in conditional NRF1 mutant testes]

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