Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Assessing the role of RIPK1 and TRIF proteins in RIPK3-transcription in MEFs

ABSTRACT: We previously have found that RIPK3 induces robust anti-viral and inflammatory transcriptional signaling in death-resistant MEFs, however the molecular mechanism that governs this transcription is not well understood. Here we found that RIPK3-depedent transcription is RIPK1 and TRIF dependent in MEFs.

ORGANISM(S): Mus musculus

PROVIDER: GSE282452 | GEO | 2025/05/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Assessing the role of RHIM proteins in RIPK3-transcription in MEFs

Project description:We previously have found that RIPK3 induces robust anti-viral and inflammatory transcriptional signaling in death-resistant MEFs, however the molecular mechanism that governs this transcription is not well understood. Here we found that RIPK3-depedent transcription is RIPK1 and TRIF dependent in MEFs.

2025-05-07 | GSE281136 | GEO

Assessing the role of RIPK1 and TRIF proteins in RIPK3-transcription in MEFs

Project description:Assessing the role of RIPK1 and TRIF proteins in RIPK3-transcription in MEFs

| PRJNA1188609 | ENA

Transcriptional profiles of PA1 teratoma cells transfected by RIPK1, RIPK2, RIPK3, or RIPK4

Project description:RIPK4 but not the related kinases RIPK1, RIPK2, and RIPK3 caused similar transcriptional changes to Wnt3a. PA1 cells were transfected by 8ug RIPK1, RIPK2, RIPK3, or RIPK4 for 48h, RNA were extracted and sequenced.

2013-02-04 | E-GEOD-43362 | biostudies-arrayexpress

Assessing RIPK3 dependent genes in death-resistant and death-susceptible MEFs encoding activatable RIPK3-system

Project description:The full transcriptional RIPK3-dependent transcriptional potential in MEFs is unknown. Here, we found that RIPK3 is a dominant driver of anti-viral and inflammatory transcription in MEFs.

2025-05-07 | GSE281061 | GEO

Assessing the role of RHIM proteins in ZIKV-induced inflammatory transcription in neurons

Project description:We previously have found that RIPK3 induces robust anti-viral and inflammatory transcriptional signaling in death-resistant MEFs in a RIPK1-and TRIF-dependent manner. We nexted wanted to assess whether this mechanism was conserved in neurons during Zika virus infection.

2025-05-07 | GSE281170 | GEO

ZBP1 and TRIF trigger lethal necroptosis in mice lacking caspase-8 and TNFR1

Project description:Necroptosis is a lytic form of cell death that is mediated by the kinase RIPK3 and the pseudokinase MLKL when caspase-8 is inhibited downstream of death receptors, toll-like receptor 3 (TLR3), TLR4, and the intracellular Z-form nucleic acid sensor ZBP1. Oligomerization and activation of RIPK3 is driven by interactions with the kinase RIPK1, the TLR adaptor TRIF, or ZBP1. In this study, we use immunohistochemistry (IHC) and in situ hybridization (ISH) assays to generate a tissue atlas characterizing RIPK1, RIPK3, Mlkl, and ZBP1 expression in mouse tissues. RIPK1, RIPK3, and Mlkl were co-expressed in most immune cell populations, endothelial cells, and many mucosal epithelia. ZBP1 was expressed in many immune populations, but had more variable expression in epithelia compared to RIPK1, RIPK3, and Mlkl. Intriguingly, expression of ZBP1 was elevated in Casp8-/- Tnfr1-/- embryos prior to their succumbing to aberrant necroptosis around embryonic day 15. ZBP1 contributed to this embryonic lethality because rare Casp8-/- Tnfr1-/- Zbp1-/- mice survived until after birth. Necroptosis mediated by TRIF contributed to the demise of Casp8-/- Tnfr1-/- Zbp1-/- pups in the perinatal period. Of note, Casp8-/- Tnfr1-/- Trif-/- Zbp1-/- mice exhibited autoinflammation and morbidity, typically within 5-7 weeks of being born, which is not seen in Casp8-/- Ripk1-/- Trif-/- Zbp1-/-, Casp8-/- Ripk3-/-, or Casp8-/- Mlkl-/- mice. Therefore, after birth, loss of caspase-8 probably unleashes RIPK1-dependent necroptosis driven by death receptors other than TNFR1.

2024-04-01 | GSE254754 | GEO

Transcriptional profiles of PA1 teratoma cells transfected by RIPK1, RIPK2, RIPK3, or RIPK4

Project description:RIPK4 but not the related kinases RIPK1, RIPK2, and RIPK3 caused similar transcriptional changes to Wnt3a.

2013-02-04 | GSE43362 | GEO

Assessing the contribution of RIPK1 in ZIKV-induced inflammatory transcription in neuorns

Project description:We previously have found that RIPK3 induces robust anti-viral and inflammatory transcriptional signaling in primary cortical neurons, however the complete molecular mechanism governing this pathway is incompletely understood. Here we show RIPK1 is required to induce RIPK3-dependent transcription in ZIKV-infected neurons.

2025-05-07 | GSE281172 | GEO

O-GlcNAcylation of RIPK1 Rescues Red Blood Cells from Necroptosis

Project description:Necroptosis is a type of cell death with excessive inflammation and organ damage in various human diseases. Although abnormal necroptosis is common in patients with neurodegenerative, cardiovascular, and infectious diseases, the mechanisms by which O-GlcNAcylation contributes to the regulation of necroptotic cell death are poorly understood. In this study, we reveal that O- GlcNAcylation of RIPK1 (receptor-interacting protein kinase1) was decreased in erythrocytes of the mouse injected with lipopolysaccharide, resulting in the acceleration of erythrocyte necroptosis through increased formation of RIPK1-RIPK3 complex. Mechanistically, we discovered that O- GlcNAcylation of RIPK1 at serine 331 in human (corresponding to serine 332 in mouse) inhibits 32 This is a provisional file, not the final typeset article phosphorylation of RIPK1 at serine 166, which is necessary for the necroptotic activity of RIPK1 and suppresses the formation of the RIPK1-RIPK3 complex in Ripk1 -/- MEFs. Thus, our study demonstrates that RIPK1 O-GlcNAcylation serves as a checkpoint to suppress necroptotic signaling in erythrocytes.

2023-06-06 | PXD042579 | Pride

Assessing RIPK3 dependent genes in death-resistant and death-susceptible MEFs encoding activatable RIPK3-system

Project description:Assessing RIPK3 dependent genes in death-resistant and death-susceptible MEFs encoding activatable RIPK3-system

| PRJNA1181905 | ENA