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The impact of RNase E C-terminal truncation mutant compared with wildtype RNase E on whole transcriptome expression profile of E. coli soft-agar plate culture

ABSTRACT: RNase E is involved in the post-transcriptional gene regulation of a broad spectrom of RNA species involving in different biological processes. Frequently RNase E cooperates with other protein components to form a RNA degradosome to regulate gene expression. Here, we aim to study the impact of RNA degradosome formation on E. coli gene expression by comparing the whole transcriptiome isolate from wildtype Rne strain and Rne C-terminal truncated strain that deleting the RNA degradosome binding domain. We choose to study the bacterial growth conditions in soft-agar culture because it is a condition more silimar to that in the gut of warm-blooded organism compared to merely liquid culture condition.Here we found different groups of transcript that was affected by Rne C-termianl truncation mutant. Altogether, our data give insight into the complex regulation of RNase E/RNA degradosome in transcripts involved in different biological processes of soft-agar growing system.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE282514 | GEO | 2025/12/04

REPOSITORIES: GEO

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Effect of different N-terminal catalytic activity of RNase E compared to full-length RNase E on whole transcriptome expression profile of E. coli soft-agar plate culture

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The impact of RNase E C-terminal truncation mutant compared with wildtype RNase E on whole transcriptome expression profile of E. coli soft-agar plate culture

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