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Ultra high-throughput single-cell transcriptome and epitope profiling of healthy human PBMCs using SCITO-seq2

ABSTRACT: This study presents comprehensive single-cell transcriptome and surface protein profiling using SCITO-seq2, an enhanced platform that improves the recovery of unique transcripts while enabling simultaneous detection of surface proteins. The analysis was conducted in two phases: first, using healthy donor PBMCs to validate technical performance, and second, by comparing samples from healthy controls, patients with childhood systemic lupus erythematosus (cSLE), and patients with CTLA4 haploinsufficiency and autoimmune infiltration (CHAI). These results demonstrate the platform’s applicability to both normal and disease states.

ORGANISM(S): Homo sapiens

PROVIDER: GSE282881 | GEO | 2026/01/04

REPOSITORIES: GEO

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