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Flavonoid and isoflavonoid biosynthetic gene expression is affected by MYB36 transcription factor deficiency in Lotus japonicus [UV-B_Experiment]

ABSTRACT: MYB36 has been identified as a new transcription factor (TF) that may be involved in isoflavonoid metabolism and stress responses in the model legume Lotus japonicus. A series of transcriptomic and metabolomic approaches have been carried out to attempt to identify the specific genes that can be under the control of MYB36 TF in this plant. For this purpose, transcriptomic data were obtained for wild type (WT) and two different lines of homozygous myb36 mutant plants, under two different stress conditions shown to be related with isoflavonoid metabolism: UV-B irradiation and salt stress. Results obtained showed a completely different and almost reciprocal picture of the global transcriptomic of the WT plants under both types of stress situations. Further comparison of up- and down- regulated genes revealed a very similar response of WT and mutant plants under UV-irradiation. In contrast, a completely different response was detected in salt stress between WT and mutant plants. None of the genes modulated by salt stress in myb36 mutants were also modulated by UV-B irradiation. Furthermore, most of the genes modulated by salt stress in the mutants corresponded to genes specifically involved in isoflavonoid but not flavonoid biosynthesis, thus illustrating on the genes that can be under the specific control of MYB36. These results identify for the first time MYB36 as specifical TF in the regulation of (iso)flavonoid biosynthesis, a class of compound specifically produced by legume plants. In addition, the results obtained revealed MYB36 as a crucial TF in the response to salt stress in legume plants.

ORGANISM(S): Lotus japonicus

PROVIDER: GSE283584 | GEO | 2026/04/24

REPOSITORIES: GEO

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Flavonoid and isoflavonoid biosynthetic gene expression is affected by MYB36 transcription factor deficiency in Lotus japonicus [Salt_Experiment]

Project description:MYB36 has been identified as a new transcription factor (TF) that may be involved in isoflavonoid metabolism and stress responses in the model legume Lotus japonicus. A series of transcriptomic and metabolomic approaches have been carried out to attempt to identify the specific genes that can be under the control of MYB36 TF in this plant. For this purpose, transcriptomic data were obtained for wild type (WT) and two different lines of homozygous myb36 mutant plants, under two different stress conditions shown to be related with isoflavonoid metabolism: UV-B irradiation and salt stress. Results obtained showed a completely different and almost reciprocal picture of the global transcriptomic of the WT plants under both types of stress situations. Further comparison of up- and down- regulated genes revealed a very similar response of WT and mutant plants under UV-irradiation. In contrast, a completely different response was detected in salt stress between WT and mutant plants. None of the genes modulated by salt stress in myb36 mutants were also modulated by UV-B irradiation. Furthermore, most of the genes modulated by salt stress in the mutants corresponded to genes specifically involved in isoflavonoid but not flavonoid biosynthesis, thus illustrating on the genes that can be under the specific control of MYB36. These results identify for the first time MYB36 as specifical TF in the regulation of (iso)flavonoid biosynthesis, a class of compound specifically produced by legume plants. In addition, the results obtained revealed MYB36 as a crucial TF in the response to salt stress in legume plants.

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