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RNA Sequencing of VeroE6 cells infected with SARS-CoV-2 in presence and absence of GYY4137

ABSTRACT: Viruses modulate various aspects of host physiology, including carbon metabolism, redox balance, and mitochondrial bioenergetics, to acquire the building blocks for replication and regulate the immune response. Understanding newer ways of how SARS-CoV-2 alters host redox metabolism, carbon metabolism, and mitochondrial health may lead to potential treatments for COVID-19. Reports have shown direct corelation of H2S metabolism with SARS-CoV-2 disease severity. However, the molecular mechanism of how H2S controls virus replication remains unknown. In this study, we have attempted to delineate the pathways altered by SARS-CoV-2 infection, using multi-omics techniques, and effect of H2S donor GYY4137 in the same. We have infected VeroE6 cells with SARS-CoV-2 (HK variant) at an MOI of 0.01 for 48 hours in presence and absence of 5 mM GYY4137. The transcriptomic profiles of uninfected, infected and treated were compared, to understand the molecular pathways underlying and to understand how H2S can modulate host metabolism to control viral replication.

ORGANISM(S): Chlorocebus sabaeus

PROVIDER: GSE283665 | GEO | 2025/04/15

REPOSITORIES: GEO

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