Unraveling age-related cellular and molecular changes associated with vestibular hair cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE283708 | GEO | 2026/02/25
REPOSITORIES: GEO
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Project description:The cochlear and vestibular sensory epithelia contain four different hair cell (HC) types for detecting sound and motion. There are substantial differences in morphology and function among them. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of HCs in the inner ear.
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Project description:The mammalian inner ear subserves auditory and vestibular sensations via highly specialized cells and proteins. We show that sensory hair cells (HCs) employ hundreds of uniquely or highly expressed proteins for processes involved in transducing mechanical inputs, stimulating sensory neurons, and maintaining structure and function of these post-mitotic cells. Our proteomic analysis of purified HCs extends the existing HC transcriptome, revealing undetected gene products and isoform-specific protein expression. Comparison with mouse and human databases of genetic auditory/vestibular impairments confirms the critical role of the HC proteome for normal inner ear function, providing a cell-specific pool of candidates for novel, important HC genes. Several proteins identified exclusively in HCs by proteomics and by immunohistochemistry map to human genetic deafness loci, potentially representing new deafness genes.
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