Mouse Spinal Cord Transcriptome Data
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE283721 | GEO | 2025/09/30
REPOSITORIES: GEO
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Project description:The spinal cord possesses precise neural circuitry to transmit messages between the brain and body. Detailed transcriptomic profiling of the developing human spinal cord has not been reported. Here, we performed single cell RNA sequencing of developing human spinal cord cells and compared these data with similar mouse spinal cord RNA sequencing datasets. The differentiation tendency of proliferative neural progenitor cells changed from neuronal to glial cells at gestational week (GW) 8 and we identified a diverse set of excitatory, inhibitory and motor neuron cell types. Human ventral neuronal differentiation occurred earlier than GW7, while DI4/5 interneurons are born between GW7–11. We identified glial cell molecular diversity and revealed that ependymal cell specification occurs before birth. We also demonstrate differences between human and mouse spinal cord, including unique cell subtypes, gene expression, neurotransmitter receptors, and glial differentiation timing. Our results offer insight into human spinal cord development.
2021-10-27 | GSE136719 | GEO
Project description:Despite the recognized importance of the spinal cord in sensory processing, motor behaviors, and/or neural diseases, the underlying organization of its cells, including both neuronal and non-neuronal clusters remain elusive. Recently, several studies have attempted to define the cellular subtypes in the spinal cord and their functional heterogeneity using single-cell and/or single-nucleus RNA-sequencing in various animal models. However, molecular evidence of cellular heterogeneity in the adult human spinal cord has not yet been established. Here, we sought to classify spinal cord neurons and glial cells from human donors using high-throughput single-nucleus RNA-sequencing. Moreover, we compared the transcriptional patterns obtained in human samples with previously published single-nucleus transcriptomic profiles of the mouse spinal cord. The functional heterogeneity among the identified cell subtypes were also explored by Gene ontology (GO) term analysis. As a result, we generated the first comprehensive transcriptomic atlas of adult human spinal cord neurons and defined 24 neuronal clusters. For glial cells, astrocytes, microglia, and oligodendrocytes were divided into ten, eight, and eleven distinct transcriptomic subclusters, respectively. The comparison with mouse transcriptomic profiles revealed an overall similarity in the neuronal composition of the spinal cord between the two species, while simultaneously highlighting some degree of heterogeneity. In summary, these results illustrate the complexity and diversity of neuronal and glial types in the human spinal cord and provide an important resource for future research to explore the molecular mechanisms underlying spinal cord physiology and diseases.
2025-06-06 | GSE205521 | GEO
Project description:The spinal cord is the critical part of the central nervous system. We performed scRNA-seq and Visum spatial RNA-seq to decipher the development of human spinal cord in the embryonic stage. Together, we reveal the dynamics of neural lineage and glial lineage during the development of human spinal cord.
2023-12-31 | GSE221692 | GEO
Project description:Mouse Spinal Cord Transcriptome Data
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Project description:This project is "Phosphoproteomic analysis of the lumbar spinal cord, a lesion site in the amyotrophic lateral sclerosis (ALS) mouse model SOD1G93A mice". The aim of this study is to clarify the phosphorylation changes by the lumbar spinal cord of SOD1G93A mice at 20w by applying proteomics technology. The goal of this study is to better understand the pathogenesis of ALS. lumbar spinal cord of SOD1G93A mice (n=5) and WT mice (n=4) were collected at 20w, and the phosphoproteomics were compared.
2022-05-19 | PXD030651 | JPOST Repository