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RNA-seq analysis of YUMM1.7 mouse melanoma cells treated with ADAR1i-124 or 5-AZA-CdR to study their effects on RNA editing

ABSTRACT: ADAR1i-124 is a small-molecule RNA editing inhibitor that can block the editase domain of ADAR1 and robustly suppress the A-to-I RNA editing on double-stranded RNAs (dsRNAs). 5-AZA-CdR is an FDA-approved DNA methyltransferase inhibitor (DNMTi) that can increase the transcription of transposable elements and thus induce more dsRNAs. To determine the efficacy of ADAR1i-124 and test whether it has overlapping targets or synergistic effects with 5-AZA-CdR, total RNA-seq analysis was performed on YUMM1.7 cells treated with 5-AZA-CdR (1 uM, 72 h), ADAR1i-124 (10 uM, 12 h) or combination of two treatments , using 0.1% DMSO as control. Immunoprecipitation (IP) with the J2 or Z22 antibodies was also adopted to enrich the dsRNA species and investigate how drugs affect the dsRNA forms and IP enrichment.

ORGANISM(S): Mus musculus

PROVIDER: GSE284082 | GEO | 2025/11/13

REPOSITORIES: GEO

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