Effect of fibulin-3 knockdown in glioblastoma stem cells

ABSTRACT: Whole transcriptome comparison of two mesenchymal GBM stem cell lines transfected with control vs fibulin-3 (EFEMP1) siRNAs. GBM stem cell lines GBM09 and GBM34 developed in-house from freshly-resected primary GBM tumors, using standard procecures (Galli et al.; Cancer Res (2002) 64:7011). Cells were transfected with two fibulin-3 or control siRNAs and collected 48h after transfection. Gene expression was quantified by RNAseq using standard procedures. The results suggest that downregulation of fibulin-3 affects NF-kB-dependent GBM gene signatures associated with angiogenesis and immunosuppression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE284269 | GEO | 2025/08/18

REPOSITORIES: GEO