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Analysis of GlycoRNAs in Glioma Cell Lines LN229 and U87

ABSTRACT: A number of studies have identified a novel class of biomolecules, namely glycosylated RNAs (glycoRNAs), which may play important roles in cellular physiology, pathology and immunity. However, whether glycoRNAs are present in glioma cells and what their functions are remain unanswered. In this study, through N-Azidoacetylmannosamine-tetraacylated (Ac4ManNAz) labeling method, glycoRNAs were enriched and purified from glioma cell lines LN229 and U87. Northern blot was performed to test the presence of glycoRNAs. It is noteworthy that we developed a sequencespecific RNA capture magnetic bead system to enrich for a specific glycoRNA such as U2, U4, and Y5. The components of glycoRNAs were analyzed and identified through RNA sequencing and chromatography-mass spectrometry.

ORGANISM(S): Homo sapiens

PROVIDER: GSE284296 | GEO | 2025/11/18

REPOSITORIES: GEO

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