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Ovary transciptomic profiles of starved surface fish and Pachón females

ABSTRACT: We performed bulk RNA sequencing on the ovaries from control and starved females of surface fish and Pachón cavefish, allowing us to study the effects of starvation on reproduction between surface fish and cavefish.

ORGANISM(S): Astyanax mexicanus

PROVIDER: GSE284646 | GEO | 2025/04/15

REPOSITORIES: GEO

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Ovary transciptomic profiles of starved surface fish and Tinaja females

Project description:We performed bulk RNA sequencing on the ovaries from control and starved females of surface fish and Tinaja cavefish, allowing us to study the effects of starvation on reproduction between surface fish and cavefish.

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Ovary transciptomic profiles of starved surface fish and Pachón females

Project description:Ovary transciptomic profiles of starved surface fish and Pachón females

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Ovary transciptomic profiles of starved surface fish and Tinaja females

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Project description:In this pioneering study, we present the first comprehensive catalog of 683 small non-coding miRNAs for Astyanax mexicanus. Focusing on an early developmental stage, miRNAs were extracted and sequenced from 24hpf embryos of surface fish and three distinct cavefish morphs (Pachón, Tinaja, and Molino). We utilized in silico analyses to predict putative 3’UTR targets of these miRNAs, revealing a unique and extensive miRNA landscape in cavefish. Small RNA sequencing identified over 100 differentially expressed miRNAs in each cave morph compared to surface fish at 24hpf, suggesting early activation of miRNA-mediated silencing pathways. Notably, a subset of miRNAs was common across all three cave morphs, constituting cave-specific miRNAs potentially instrumental in cave adaptation. To unravel the functional implications of these cave-specific miRNAs, we analyzed their predicted target genes. Gene Ontology (GO) term analysis unveiled pathways which align with known adaptations in cavefish, primarily affecting development and metabolism. Further, cross-validating with a sample mRNAseq data from Pachón and surface fish also strongly suggested impact of these miRNAs on cave adaptation associated pathways. This study establishes a foundation for exploring miRNA-mediated gene regulation in cavefish, shedding light on their potential role in regulating early developmental and metabolic adaptations crucial for troglomorphic features. The comprehensive miRNA catalog provided will also guide future investigations into the intricate world of miRNA-mediated evolution in cave-adapted species.

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Single cell RNA sequencing reveals the functions of ovary in Astyanax mexicanus

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Astyanax mexicanus strain:Pachon cavefish and Surface fish

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Comparative transcriptome analysis of wild and lab populations of Astyanax mexicanus uncovers differential effects of environment and morphotype on gene expression

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