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S100A4 inhibition alters pre-metastatic niche formation in 4T1 lung metastasis model

ABSTRACT: Cancer metastasis poses a major clinical challenge and is responsible for ~90% of cancer-associated deaths. However, progress in treating metastatic disease is limited. S100A4/FSP1 has long been associated with cancer metastasis, including breast cancer. In this study, we report preclinical efficacy data of a novel S100A4 blocking antibody that suppresses breast cancer metastasis. 4T1 and MMTV-PyMT breast cancer metastasis to the lung is greatly attenuated by S100A4 blocking antibody treatment. Immunohistochemistry analysis shows that neutrophil infiltration is significantly reduced in the anti-S100A4 treated mice. Single-cell RNA sequence analysis of the premetastatic niche treated with either isotype control or anti-S100A4 antibody from the 4T1 model show that NK and T cell activation in the lung. In this study, we report that S100A4 promotes premetastatic niche formation by promoting neutrophil infiltration and suppressing the function of cytotoxic NK and T cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE284668 | GEO | 2026/05/15

REPOSITORIES: GEO

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