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Trpv4 specifically inhibits p53-driven Dkk1 and Sost secretion in a novel Kcnk2+ osteolineage subpopulation to rejuvenate the aged osteo-brain axis

ABSTRACT: This comprehensive study aims to elucidate the multi-dimensional transcriptional and epigenetic mechanisms underlying osteogenic lineage fate remodeling and bone homeostasis during aging, mechanical intervention, and targeted pharmacological therapy. Bulk RNA-sequencing (RNA-seq) was performed to evaluate global transcriptomic alterations in both brain and bone tissues across aging, treadmill exercise, and bone-targeted drug treatments. To complement the tissue-level analysis, single-cell RNA sequencing (scRNA-seq) of mouse bone models was integrated to map osteogenic heterogeneity at single-cell resolution. Additionally, CUT&Tag profiling was utilized to delineate the direct genome-wide binding landscapes of downstream transcriptional regulators orchestrating this lineage transition.

ORGANISM(S): Mus musculus

PROVIDER: GSE285020 | GEO | 2025/04/23

REPOSITORIES: GEO

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Trpv4 specifically inhibits p53-driven Dkk1 and Sost secretion in a novel Kcnk2+ osteolineage subpopulation to rejuvenate the aged osteo-brain axis

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