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Gene expression and chromatin accessibility in human embryonic stem cells with partial deletion of PDHB during DE differentiation [RNA-seq]

ABSTRACT: We established a PDHB heterozygous cell line in human embryonic stem cell HUES8. Then we performed definitive endoderm (DE) differentiation using both WT and PDHB partial deletion cell lines, and assessed gene expression and chromatin accessibility changes during this process by RNA-seq and ATAC-seq. Differentially expressed genes and regions with altered chromatin accessibility showed that in the PDHB-deleted cell line, the efficiency of endoderm differentiation was significantly inhibited. This alteration primarily occurred through changes in chromatin accessibility at BAF complex binding sites on DNA, which is a major ATP-dependent chromatin remodeling complex. And we also detected the SMAD2/3 binging by ChIP-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE285135 | GEO | 2025/11/24

REPOSITORIES: GEO

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