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Single-cell RNA sequencing combined with multiplex immunofluorescence probes the role of MFAP5+ fibroblasts in the microenvironment of pancreatic ductal adenocarcinoma

ABSTRACT: we employed single-cell RNA sequencing to examine the biological characteristics of MFAP5+ fibroblasts in PDAC and their interaction with vascular endothelial cells within tumors. We then utilized a proposed temporal sequencing analysis technique to infer the evolution of cellular subtypes of cancer-associated fibroblasts. To verify our hypothesis, we employed a multiplex immunofluorescence technique to observe the spatial distribution of MFAP5+ fibroblasts and endothelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE285264 | GEO | 2024/12/28

REPOSITORIES: GEO

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