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Spatially resolved m6A profiling using m6A-ARTR-DBiT [spatial]

ABSTRACT: N6-methyladenosine (m6A) on RNA plays diverse regulatory roles, yet its spatial distribution within tissues remains largely unexplored. Here, we develop a spatial m6A profiling assay leveraging reverse transcription-based detection and deterministic barcoding in tissue (m6A-ARTR-DBiT), enabling spatially resolved sequencing of m6A within the native tissue context. Application of m6A-ARTR-DBiT to embryonic and adult mouse brain generated high-resolution whole transcriptome spatial m6A maps, offering insights into region-specific m6A modification.

ORGANISM(S): Mus musculus

PROVIDER: GSE285304 | GEO | 2026/01/14

REPOSITORIES: GEO

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