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The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling

ABSTRACT: We identified a key regulator of the rice leaf angle and plant architecture, OsWRKY36, from a rice oswrky mutant library. OsWRKY36 is highly expressed in the leaf lamina joint and promotes cell growth and expansion in adaxial parenchyma cells, leading to a greater leaf angle.

ORGANISM(S): Oryza sativa

PROVIDER: GSE285338 | GEO | 2025/01/01

REPOSITORIES: GEO

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The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling [DAP-seq]

Project description:WRKY transcription factors constitute a crucial protein superfamily that plays a vital role in orchestrating diverse responses to environmental stimuli in plants. Although considerable research has been conducted on the stress resistance mechanisms associated with WRKYs, little is known about the roles of WRKYs in regulating plant growth and development. Here, we identified a key regulator of the rice leaf angle and plant architecture, OsWRKY36, from a rice oswrky mutant library. OsWRKY36 is highly expressed in the leaf lamina joint and promotes cell growth and expansion in adaxial parenchyma cells, leading to a greater leaf angle. A mechanistic investigation revealed that OsPUB24, an E3 ubiquitin ligase that is inhibited by brassinosteroid (BR), interacts with and facilitates the ubiquitination and degradation of OsWRKY36. Genetic evidence indicated that OsWRKY36 acts downstream of OsPUB24 to regulate the BR-induced increase in the leaf angle. Furthermore, OsWRKY36 physically interacts with DLT, a positive regulator of BR, and cooperatively activates the expression of the BR response gene OsBZR4. Thus, BR facilitates the degradation of OsPUB24, leading to enhanced stability of the OsWRKY36 protein. Consequently, a substantial quantity of OsWRKY36 interacts with DLT, thereby inducing the expression of OsBZR4 and ultimately exerting a positive regulatory effect on BR-induced leaf inclination. Collectively, our findings reveal the mechanism underlying the regulation of leaf inclination and plant architecture by the BR-OsPUB24-OsWRKY36 and DLT-OsWRKY36-OsBZR4 modules and thus provide an innovative strategy for optimizing crop production by manipulating the BR signalling pathway.

2025-12-01 | GSE310494 | GEO

The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling [ChIP-Seq]

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Spatiotemporal resolved leaf angle establishment improves rice grain yield via controlling population density [mRNA]

Project description:Leaf angle is mainly determined by the lamina joint (LJ), and contributes to ideal crop architecture for high yield. Here, we dissected five successive stages with distinct cytological features of LJs spanning organogenesis to leaf angle formation, and obtained the underlying stage-specific mRNAs and small RNAs, which well explained the cytological dynamics during LJ organogenesis and leaf angle plasticity. Combining the gene coexpression correlation with high-throughput promoter analysis, we identified a set of transcription factors determining the stage- and/or cytological structure-specific profiles. The functional studies of these TFs demonstrated that cytological dynamics determined leaf angle, and the knockout rice of these TFs with erect leaves significantly enhanced yield by maintaining the proper tiller number under dense planting. This work revealed the high-resolution mechanisms how the cytological dynamics of LJ determined the leaf erectness, and served as a valuable resource to remodel rice architecture for high yield via controlling population density.

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Spatiotemporal resolved leaf angle establishment improves rice grain yield via controlling population density [miRNA-seq]

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Genome-wide gene expression profiling of rice Zhonghua11 and ila1 lamina joint

2012-05-01 | GSE33361 | GEO

Genome-wide gene expression profiling of rice Zhonghua11 and ila1 lamina joint

Project description:The leaf lamina joint joins the rice leaf blade and sheath, contributing significantly to the leaf angle trait. A more erect leaf facilitates the penetration of sunlight, enhancing photosynthetic efficiency and occupying less space in dense planting. Genetic screening found a mutant increased leaf angle1, ila1 from rice T-DNA insertional mutants library. We used microarrays to detail the transcriptional profile changes in the mutant ila1 lamina joint. Two biological replicate sample of leaf lamina joints from the ila1 and wild-type plants at tillering stage were collected for RNA extraction. Total RNAs were isolated from each replicate via the TRIzol method (Invitrogen) and used in target synthesis for the Rice Genome Array from Affymetrix. The microarray analyses were performed through following standard protocols (Affymetrix).

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Expression analysis of genes in the lamina joint of lc1 mutant compared with that of wild-type

Project description:The angle of rice leaf inclination is an important agronomic trait and closely related to the yield and architecture of crops. Through genetic screening, a rice gain-of-function mutant leaf inclination1, lc1, was identified . Phenotypic analysis confirmed the exaggerated leaf angels of lc1 due to the stimulated cell elongation at the collar.In this series, we compare the transcriptome of zhonghua11 and lc1 collar.

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The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling

Project description:The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling

| PRJNA1203189 | ENA

The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling [ChIP-Seq]

Project description:The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling [ChIP-Seq]

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The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling [DAP-seq]

Project description:The transcription factor OsWRKY36 regulates lamina joint development and the leaf angle in rice by modulating brassinosteroid signalling [DAP-seq]

| PRJNA1366433 | ENA

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